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Chiralpakad h chiral column

Manufactured by Daicel
Sourced in Japan

The ChiralpakAD-H is a chiral column used for the separation and analysis of enantiomers. It is designed for high-performance liquid chromatography (HPLC) applications. The column consists of a cellulose-based stationary phase that enables the resolution of chiral compounds.

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2 protocols using chiralpakad h chiral column

1

Analytical Techniques for Compound Characterization

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Optical rotations were measured on
a Rudolph Autopol IV polarimeter (Hackettstown). Ultraviolet (UV)
spectra were obtained using a double beam spectrophotometer (UH5300,
Hitachi High-Technologies, Tokyo, Japan). Infrared (IR) spectra were
obtained on a Shimadzu Fourier transform infrared spectrometer using
KBr pellets. High-resolution electrospray ionization mass spectra
(HRESIMS) were measured on an Exactive Orbitrap mass spectrometer
with a HESI ion source (ThermoFisher Scientific, Bremen, Germany).
Nuclear magnetic resonance (NMR) spectra were recorded with a Bruker
Avance III 600 MHz spectrometer (Bruker, Karlsruhe, Germany) with
tetramethylsilane (TMS) as an internal standard. Circular dichroism
(CD) spectra were measured with an Applied Photophysics spectrometer
(Chirascan, New Haven). Silica gel (200–300 mesh), Sephadex
LH-20 (GE Healthcare), and reverse phase (RP)-18 gel (20–45
μm, FuJi) were used for column chromatography. Preparative or
semipreparative high-performance liquid chromatography (HPLC) was
performed on an Agilent 1260 liquid chromatography system equipped
with Zorbax SB-C18 columns (5 μm, 9.4 mm × 150 mm or 21.2
mm × 150 mm). Chiral separation was carried out on a Chiralpak
AD-H chiral column (5 μm, 250 × 4.6 mm, Daicel).
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2

Analytical Techniques for Compound Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
UV spectra were obtained by using a Double Beam Spectrophotometer UH5300 (Hitachi High-Technologies, Tokyo, Japan). IR spectra were obtained on a Shimadzu Fourier Transform Infrared spectrometer using KBr pellets. NMR spectra were recorded with a Bruker Avance III 600 MHz spectrometer (Bruker, Karlsruhe, Germany). Optical rotations were measured on a Rudolph Autopol IV polarimeter (Hackettstown, NJ, USA). HRESIMS were measured on an Agilent (Santa Clara, CA, USA) 6200 Q-TOF MS system. Circular dichroism (CD) spectra were measured with an Applied Photophysics spectrometer (Chirascan, New Haven, CT, USA). HPLC was performed on an Agilent 1260 liquid chromatography system equipped with Zorbax (Santa Clara, CA, USA) SB-C18 columns (5 μm, 9.4 mm × 150 mm or 21.2 mm × 150 mm). Sephadex LH-20 (GE Healthcare, Chicago, IL, USA), Silica gel (200–300 mesh), and RP-18 gel (20–45 μm, FuJi, Tokyo, Japan) were used for column chromatography (CC). Chiral separation was carried out on a Chiralpak AD-H chiral column (5 μm, 250 × 4.6 mm; Daicel, Osaka, Japan).
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