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Legendplex t helper cytokine panel

Manufactured by BioLegend
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Sourced in United States

The LEGENDplex T helper cytokine panel is a multiplex bead-based assay designed to simultaneously quantify multiple cytokines secreted by T helper cells. The panel allows for the measurement of several analytes in a single sample, providing a comprehensive assessment of the T helper cell response.

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Lab products found in correlation

Non radioactive cytotoxicity assay, by Promega (1 mentions)

Close spelling variants of this product

LEGENDplex (292 citations) LEGENDplex panels (5 citations) Legendplex T Helper Cytokine Panel Version 2 (3 citations) Human T Helper Cytokine Panel LEGENDplex bead-based immunoassay (3 citations) LEGENDplex Human T helper Cytokine panel (3 citations) LEGENDplex mouse T helper cytokine panel (3 citations) LegendPlex™ T Helper Cytokine Version 2 and Proinflammatory Chemokine panel kits (2 citations)

2 protocols using legendplex t helper cytokine panel

1

Assessing IFN-γ-producing T Cells in Splenocytes

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IFN-γ-producing T cells in isolated splenocytes was assessed by ELISpot assay.11 (link),32 (link) A mouse TWIST1 peptide library (of 49 peptides generated as 15-mers overlapping by 11 amino acids), gp70-AH1 (SPSYVYHQF), and ovalbumin (OVA257–264) were synthesized by GL Biochem (Shanghai). Cytokine concentrations in co-culture supernatant were measured by LEGENDplex T helper cytokine panel (BioLegend). Cytotoxic effect of purified T cells against AB1 cells was determined using a nonradioactive cytotoxicity assay (Promega) according to the manufacturer’s instructions.
Tan Z., Chiu M.S., Yan C.W., Wong Y.C., Huang H., Man K, & Chen Z. (2020). Antimesothelioma Immunotherapy by CTLA-4 Blockade Depends on Active PD1-Based TWIST1 Vaccination. Molecular Therapy Oncolytics, 16, 302-317.
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2

Bronchoalveolar Lavage and Lung Lymphocyte Isolation

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Bronchioalveolar lavage (BAL) and isolation of cells from the bronchoalveolar lavage fluid (BALF) was performed as previously described [42 (link)]. Cytokine levels in the BALF were determined using BioLegend’s LEGENDplex T Helper Cytokine Panel (BioLegend, San Diego, CA, USA), according to the manufacturers’ instructions. Lung lymphocytes were isolated as previously described [42 (link)].
Heine S., Aguilar-Pimentel A., Russkamp D., Alessandrini F., Gailus-Durner V., Fuchs H., Ollert M., Bredehorst R., Ohnmacht C., Zissler U.M., Hrabě de Angelis M., Schmidt-Weber C.B, & Blank S. (2022). Thermosensitive PLGA–PEG–PLGA Hydrogel as Depot Matrix for Allergen-Specific Immunotherapy. Pharmaceutics, 14(8), 1527.
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