Dab color kit

Manufactured by Zhongshan Golden Bridge Biotechnology

Sourced in China

The DAB color kit is a laboratory reagent used for immunohistochemical staining. It provides a brown chromogenic reaction to visualize target proteins in tissue samples.

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Lab products found in correlation

Lps escherichia coli o55 b5, by Merck Group (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Radioimmunoprecipitation assay (ripa), by Beyotime (1 mentions)

Spelling variants of this product

DAB kit (38 citations) DAB Horseradish Peroxidase Color Development Kit (7 citations) DAB color development kit (2 citations)

3 protocols using dab color kit

Molecular Mechanisms of Inflammation

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LPS (Escherichia coli O55: B5) was purchased from Sigma (St. Louis, MO, USA). Bifidobacterium microcapsules were purchased from Guangzhou Institute of Microbiology (Guangzhou, China). The NF-kB p65 immunohistochemistry primary antibody (rabbit antirat) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The TLR2 immunohistochemistry primary antibody (rabbit anti-rat) was purchased from GenWay Biotech, Inc. (San Diego, CA, USA). TLR4 immunohistochemistry primary antibody (rabbit anti-rat) was purchased from Abcam (Cambridge, UK). The universal IgG antibody-horseradish peroxidase multimers (goat anti-rabbit) and DAB color kit were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China).

Zhou W., Lv H., Li M.X., Su H., Huang L.G., Li J, & Yuan W.M. (2015). Protective effects of bifidobacteria on intestines in newborn rats with necrotizing enterocolitis and its regulation on TLR2 and TLR4. Genetics and molecular research : GMR, 14(3).

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Immunohistochemical Analysis of ENO1 and FBXW7

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A total of 50 samples of colorectal cancer tissues and corresponding adjacent non-cancerous tissues were obtained from patients undergoing surgical excision of tumors in Qilu Hospital of Shandong University (Jinan, China). The samples were fixed with 10% formalin and embedded in paraffin and then were sliced into 5-µm sections. The sections were deparaffinized in xylene and rehydrated through graded alcohol. After incubation in 0.3% H₂O₂ and blocked with 10% goat serum, the sections were incubated overnight at 4 °C with antibodies against ENO1 (1:200) and against FBXW7 (1:300). Subsequently, the sections were treated with secondary antibody for 30 min, developed with diaminobenzidine (DAB) substrate (DAB Color Kit (Beijing Zhong-shan Golden Bridge Biotechnology)) and counterstained with hematoxylin. Staining was observed in five randomly selected fields in the high-power microscope. The staining intensity was based on the average percentage of positive cells. The scoring results were analyzed by two investigators.

Zhan P., Wang Y., Zhao S., Liu C., Wang Y., Wen M., Mao J.H., Wei G, & Zhang P. (2015). FBXW7 negatively regulates ENO1 expression and function in colorectal cancer. Laboratory investigation; a journal of technical methods and pathology, 95(9), 995-1004.

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Extraction and Preparation of TSG from Polygonum multiflorum

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UCP-4 polyclonal antibody (Santa Cruz Biotechnology Inc., Dallas, TX, USA); SABC immunohistochemistry kit, 5-HT rabbit monoclonal antibody, 5-HT2A rabbit monoclonal antibody, 5-HTT rabbit monoclonal antibody, DAB color kit (Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China); polyvinylidene fluoride (PVDF) membrane (Millipore Corp., Billerica, MA, USA); hydrated trichloroacetaldehyde (Shanghai Chemical Reagent Research Institute Co., Ltd., Shanghai, China); RIPA (Beyotime Biotechnology, Jiangsu Province, China); and Bioshine ChemiQ 4600mini chemiluminescence imaging system (Bioshine, Shanghai, China)were used. Cooked Polygonum multiflorum Thumb was purchased from Longyuan Pharmaceutical Co., Ltd. (Hunan Province, China) with batch No. 20150901, and TSG extracts from Polygonum multiflorum were made in the Chinese Medicine Research Center, Hunan University of Medicine (Huaihua, Hunan Province, China). Polygonum multiflorum was extracted twice by 70% ethanol via reflux extraction, then dried in a basic environment, and concentrated in a vacuum. The extracted TSG (6.25, 12.50, and 25.00 g) was dissolved in 50 mL of water to prepare 125, 250, and 500 mg/mL solutions.

Yi C.A., Wang J., Wang Y, & Wu X.Y. (2019). Neuroprotection by 2,3,5,4ʹ-tetrahydroxystilbene-2-O-β-D-glucoside extracts from Polygonum multiflorum against cerebral ischemia/reperfusion injury through the 5-hydroxytryptamine/5-hydroxytryptamine receptor pathway. Neuropsychiatric Disease and Treatment, 15, 1429-1438.

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