400nmr apparatus

Structural elucidation of the isolated molecules was performed using an Agilent Technology (USA) 400NMR apparatus. Hydrogen and carbon 13 spectra were recorded on a BRUKER TOPSPIN 2.1 (1H-NMR: 400 and 600 MHz and 13C-NMR: 125 MHz) NMR spectrometry system using the Bruker pulse sequence standard. Two-dimensional measurements (H-H COSY, HMBC, HMQC) were obtained on the same instrument with the usual pulse sequences. Peaks were visualized from ACD/NMR Processor Academic Edition software.

Hispiduloside structural elucidation was performed using an Agilent Technology (Santa Clara, CA, USA) 400 NMR apparatus. Hydrogen and carbon 13 spectra were recorded on a BRUKER TOPSPIN 2.1 (¹H-NMR: 400 and 600 MHz and ¹³C-NMR: 125 MHz) NMR spectrometry system using the Bruker pulse sequence standard (Bruker, Billerica, MA, USA).

Structural elucidation of the isolated molecules was performed using an Agilent Technology (Santa Clara, CA, USA) 400NMR apparatus. Hydrogen and carbon 13 spectra were recorded on a BRUKER TOPSPIN 2.1 (1H-NMR: 400 and 600 MHz and 13C-NMR: 125 MHz) NMR spectrometry system using the Bruker pulse sequence standard (Bruker, Billerica, MA, USA). Two-dimensional measurements (homonuclear correlation spectroscopy (H-H COSY), Heteronuclear Multiple Bond Correlation (HMBC), Heteronuclear Multiple Quantum Coherence (HMQC)) were obtained on the same instrument with the usual pulse sequences. Peaks were visualized from ACD/NMR Processor Academic Edition software version 12.01.
Both SFCO₂E and pectolinarigenin, an isolated flavonoid, were used to evaluate the pharmacological activities. Pectolinarigenin was isolated by a combination of one run of the HSCCC and size exclusion chromatography (Sephadex LH-20).