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Silica gel 60 f254 plates

Manufactured by Fujifilm
Sourced in Japan

Silica gel 60 F254 plates are thin-layer chromatography (TLC) plates used for the separation and identification of chemical compounds. The silica gel 60 F254 substrate provides a surface for the adsorption and separation of analytes, while the fluorescent indicator F254 allows for the visualization of separated compounds under ultraviolet light.

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2 protocols using silica gel 60 f254 plates

1

Spectroscopic Analysis of Organic Compounds

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Nuclear magnetic resonance (NMR) spectra were recorded using a JNM-ECS400 NMR spectrometer (JEOL Ltd., Tokyo, Japan) operated at 400 MHz (1H) with tetramethylsilane as internal standard. Gas chromatograph–mass spectrometry (GC–MS) analyses were performed using a Shimadzu QP 2010 mass spectrometer (Shimadzu, Kyoto, Japan) equipped with a Shimadzu GC2010 gas chromatography system and an AOC-20i autosampler. High-performance liquid chromatography (HPLC) analyses were performed using an LC-20AD pump equipped with an SPD-20A UV/VIS detector (Shimadzu Corporation). Column chromatography was run on silica gel (Silica gel 60; Nacalai Tesque Inc., Kyoto, Japan). Precoated thin-layer chromatography (TLC) was performed on Silica gel 60 F254 plates (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan). Electron ionization–mass spectrometry (EI–MS) spectra were obtained with a JMS-700 MStation mass spectrometer (JEOL Ltd.). The optical rotations were measured on a DIP-1000 polarimeter (JASCO Corporation, Hachioji, Tokyo, Japan).
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2

Detailed Silica Gel Column Chromatography

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Silica gel column chromatography was performed with Silica gel 60 (20–230 mesh; Nacalai Tesque Inc., Kyoto, Japan) or Wakogel C-300 HG (Fujifilm Wako Pure Chemical Corporation, Osaka, Japan). Precoated TLC was performed on Silica gel 60 F254 plates (Fujifilm Wako Pure Chemical Corporation). NMR spectra were recorded using a JNM-ECS400 NMR spectrometer (JEOL Ltd., Akishima, Tokyo, Japan), which was operated at 400 MHz (1H) and 100 MHz (13C). Tetramethylsilane (internal standard of NMR spectrometry), deuterated chloroform (CDCl3), and deuterated methanol (CD3OD) were obtained from Euriso-Top (Saint-Aubin, France). Optical rotations of compounds were measured on a DIP-1000 polarimeter (JASCO Corporation, Hachioji, Tokyo, Japan). EI–MS spectra were obtained with a JMS-700 MStation mass spectrometer (JEOL Ltd.). ChemDraw software (PerkinElmer Inc., Waltham, MA, USA) was used to draw chemical structures.
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