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Mouse ip 10 elisa kit

Manufactured by Thermo Fisher Scientific
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The Mouse IP-10 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) used for the measurement of mouse Interferon-gamma Induced Protein 10 (IP-10) in cell culture supernatants, serum, and plasma samples. The kit includes a 96-well pre-coated plate, standards, and necessary reagents for performing the assay.

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Lab products found in correlation

Recombinant mouse ifn γ, by BioLegend (1 mentions) Fludarabine, by Bio-Techne (1 mentions) Fluorstar optima plate reader, by BMG Labtech (1 mentions)

Spelling variants of this product

Mouse ELISA kits (105 citations) ELISAs (35 citations) IP-10 (18 citations)

2 protocols using mouse ip 10 elisa kit

1

IFN-γ-Induced CXCL10 Production

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2 x 104 cells were incubated with Fludarabine (Tocris Bioscience) at 37C for two hours in complete culture media. Then cells were stimulated with 1ng/ml of recombinant mouse IFN-γ (Biolegend) for 24 hours. Mouse CXCL10 protein concentration was measured from supernatants mouse IP-10 ELISA kit (Invitrogen) according to manufacturer’s protocol.
Tan T.G., Zybina Y., McKenna C., Olow A., Rukmini S.J., Wong M.T., Sadekova S., Chackerian A, & Bauché D. (2022). SPATA2 and CYLD inhibit T cell infiltration into colorectal cancer via regulation of IFN-γ/STAT1 axis. Frontiers in Oncology, 12, 1016307.
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2

Mouse IP-10 ELISA Quantification

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Complete RPMI media was conditioned in LKR13 K or LKR13 KA cells for 48 hours. Conditioned media was removed and stored at −80°C as 1ml aliquots. Followed manufacturer instructions for Mouse IP-10 ELISA kit (Invitrogen, BMS6018). Briefly, ELISA strips were prewashed twice. 100μl undiluted tissue culture media was added per sample. 50μl biotin-conjugated antibody was added per well and incubated 2h. Each well was then washed 6 times. 100μl Streptavidin-HRP was added and incubated for 1h while shaking. Each well was again washed 6 times. Added 100μl TMB substrate for 10min while slowly shaking. Added 100μl STOP solution then immediately read absorbancy at 450nm using a fluorSTAR Optima plate reader (BMG Labtech). All steps and incubations were performed at room temperature.
Frossard M., Joukhadar C., Erovic B.M., Dittrich P., Mrass P.E., Van Houte M., Burgmann H., Georgopoulos A, & Müller M. (2000). Distribution and Antimicrobial Activity of Fosfomycin in the Interstitial Fluid of Human Soft Tissues. Antimicrobial Agents and Chemotherapy, 44(10), 2728-2732.
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