For RNA isolation, mouse embryos were washed in ice-cold PBS and homogenized in Trizol reagent (Invitrogen). Upon addition of chloroform, total RNA was isolated from water phase and purified with PureLink RNA Mini Kit columns (Ambion) and PreLink DNase (Invitrogen). cDNA was reverse transcribed from isolated RNA with Omniscript RT kit (QIAGEN) using oligo-dT primers (Thermo Scientific). Quantitative PCR was performed with SYBR Green I Master reagent (Roche) using Lightcycler 480 system (Roche). The expression of target genes was calculated by E-method (Tellmann, 2006 ) and normalized to the housekeeping gene (β-actin). All primer sequences are listed in Table S1 .
Prelink dnase
Manufactured by Thermo Fisher Scientific
PreLink DNase is a laboratory reagent used to remove DNA from RNA samples. It functions by hydrolyzing the phosphodiester bonds in DNA, thereby degrading the DNA present in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Purelink rna mini kit columns, by Thermo Fisher Scientific (2 mentions)
Sybr green 1 master reagent, by Roche (2 mentions)
Oligo dt primer, by Thermo Fisher Scientific (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Lightcycler 480 system, by Roche (2 mentions)
Omniscript rt kit, by Qiagen (2 mentions)
2 protocols using prelink dnase
1
RNA Isolation and qPCR Analysis
2
RNA Isolation and qPCR Analysis
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For RNA isolation, mouse embryos were washed in ice-cold PBS and homogenized in Trizol reagent (Invitrogen). Upon addition of chloroform, total RNA was isolated from water phase and purified with PureLink RNA Mini Kit columns (Ambion) and PreLink DNase (Invitrogen). cDNA was reverse transcribed from isolated RNA with Omniscript RT kit (QIAGEN) using oligo-dT primers (Thermo Scientific). Quantitative PCR was performed with SYBR Green I Master reagent (Roche) using Lightcycler 480 system (Roche). The expression of target genes was calculated by E-method (Tellmann, 2006 ) and normalized to the housekeeping gene (β-actin). All primer sequences are listed in Table S1 .
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