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Superscript 2 reverse transcriptase for qpcr

Manufactured by Thermo Fisher Scientific
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SuperScript II Reverse Transcriptase is a recombinant reverse transcriptase enzyme used for the conversion of RNA to cDNA for quantitative PCR (qPCR) applications. It has been engineered for improved thermal stability and reduced RNase H activity.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (2 mentions) Quantitect primer, by Qiagen (2 mentions) Sybr green pcr master mix, by Thermo Fisher Scientific (2 mentions) Abi prism 7900 sequence detection system, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Superscript II (2725 citations) Reverse transcriptase (531 citations) SuperScript reverse transcriptase (349 citations) Superscript II reverse (55 citations) SuperScript II transcriptase (22 citations) Reverse transcriptase II (16 citations) Transcriptase II (5 citations)

2 protocols using superscript 2 reverse transcriptase for qpcr

1

Quantitative Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total cellular RNAs were extracted using QIAGEN RNeasy Mini Kit (# 74104) according to the manufacturer’s instructions, and RNA purity was confirmed by analysis of the ratio of absorbance at 260 nm to that at 280 nm absorption. cDNAs were prepared from 1 g total RNAs using SuperScript II Reverse Transcriptase for qPCR (Invitrogen, #18064022) according to the manufacturer’s instructions. qRT-PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems, # 4344463) and QuantiTect Primer (QIAGEN) in an ABI PRISM 7900 Sequence Detection System (Applied Biosystems). Relative mRNA expression was calculated using the comparative Ct method after normalization to a loading control. Samples were run in triplicates with a primer-limited probe for the reference gene. Primers are provided in Key Resources Table.
Muraleedharan R., Gawali M.V., Tiwari D., Sukumaran A., Oatman N., Anderson J., Nardini D., Bhuiyan M.A., Tkáč I., Ward A.L., Kundu M., Waclaw R., Chow L.M., Gross C., Rao R., Schirmeier S, & Dasgupta B. (2020). AMPK-Regulated Astrocytic Lactate Shuttle Plays a Non-Cell-Autonomous Role in Neuronal Survival. Cell reports, 32(9), 108092.
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2

Quantitative Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total cellular RNAs were extracted using QIAGEN RNeasy Mini Kit (# 74104) according to the manufacturer’s instructions, and RNA purity was confirmed by analysis of the ratio of absorbance at 260 nm to that at 280 nm absorption. cDNAs were prepared from 1 g total RNAs using SuperScript II Reverse Transcriptase for qPCR (Invitrogen, #18064022) according to the manufacturer’s instructions. qRT-PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems, # 4344463) and QuantiTect Primer (QIAGEN) in an ABI PRISM 7900 Sequence Detection System (Applied Biosystems). Relative mRNA expression was calculated using the comparative Ct method after normalization to a loading control. Samples were run in triplicates with a primer-limited probe for the reference gene. Primers are provided in Key Resources Table.
Muraleedharan R., Gawali M.V., Tiwari D., Sukumaran A., Oatman N., Anderson J., Nardini D., Bhuiyan M.A., Tkáč I., Ward A.L., Kundu M., Waclaw R., Chow L.M., Gross C., Rao R., Schirmeier S, & Dasgupta B. (2020). AMPK-Regulated Astrocytic Lactate Shuttle Plays a Non-Cell-Autonomous Role in Neuronal Survival. Cell reports, 32(9), 108092.
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