Rabbit anti zip14

Neurosensory retina protein lysates were extracted using Laemmli SDS lysis buffer supplemented with protease/phosphatase inhibitor mixture and phenylmethylsulfonyl fluoride (PMSF) (Cell Signaling Technology, Danvers, MA, USA). Lysates were treated and run as described previously.^{25 (link)} Imaging was done using GE Amersham Imager 600 (GE Healthcare, Chalfont St. Giles, UK). FIJI software was used for band densitometry.^{24 (link)} Primary antibodies used were as follows: rat anti-transferrin receptor (Serotec), rabbit anti-Dmt1 (NRAMP24-A; Alpha Diagnostic International, San Antonio, TX, USA), rabbit anti-Zip8 (ThermoScientific, Philadelphia, PA, USA), rabbit anti-Zip14 (ThermoScientific), rabbit anti-Fpn (Pierce, Chicago, IL, USA), rabbit anti-ferritin (E17) (P. Arosio), and mouse anti-beta actin (ab8226; Abcam). Secondary antibodies used were as follows: IRDye 680RD donkey anti-rabbit (LI-COR P/N 926-68072), donkey anti-mouse (LI-COR P/N 925-68070), and donkey anti-rat (LI-COR P/N 925-68076). All primary antibodies were used at a 1:1000 dilution and all secondary antibodies were used at a 1:5000 dilution. β-Actin served as an internal control.