Serum samples were randomly selected from the cases and controls at the same ratio as was used for the Proseek® plates (35 ovarian cancer and 53 healthy control). Enzyme-linked immunosorbent assays (ELISA) for HE4 (R&D Systems), EGF (Raybiotech and R&D Systems), and MSLN (R&D Systems and Biolegend) were performed following manufacturer’s instructions.
Epidermal growth factor (egf)
Manufactured by RayBiotech
Sourced in United States
Epidermal Growth Factor (EGF) is a protein that stimulates cell growth and differentiation. It is a key signaling molecule involved in the regulation of various cellular processes. EGF plays a crucial role in the development and maintenance of tissues, particularly in the skin and other epithelial structures.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant egfr, by RayBiotech (2 mentions)
Mda mb 231, by American Type Culture Collection (2 mentions)
Hb egf, by RayBiotech (2 mentions)
A2780cis, by Merck Group (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Caov3, by American Type Culture Collection (1 mentions)
Nih ovcar3, by American Type Culture Collection (1 mentions)
Mda mb 468, by American Type Culture Collection (1 mentions)
Bt 20, by American Type Culture Collection (1 mentions)
Leptin, by RayBiotech (1 mentions)
Vegf a, by RayBiotech (1 mentions)
Hepatocyte growth factor (hgf), by RayBiotech (1 mentions)
Ang 2, by RayBiotech (1 mentions)
Angiogenin, by RayBiotech (1 mentions)
Tgf β1, by R&D Systems (1 mentions)
Vegf c, by R&D Systems (1 mentions)
Ang 1, by R&D Systems (1 mentions)
Basic fibroblast growth factor (bfgf), by RayBiotech (1 mentions)
Pdgf bb, by RayBiotech (1 mentions)
Tgf α, by RayBiotech (1 mentions)
5 protocols using epidermal growth factor (egf)
1
Ovarian Cancer Biomarker Assays
2
Cell Line Characterization and Therapeutic Antibody Sourcing
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The OC cell lines (SKOV3, CaOV3, PA1, NIH-OVCAR3, and SW126) and the breast cancer cell line (MDA-MB-231) were obtained from American Type Culture Collection (Manassas, VA, USA) and propagated at 37°C under 5% CO 2 in culture media conditions as described in manufacture's protocol. OC cells (A2780 and A2780cis) were purchased from Sigma-Aldrich (St. Louis, MO). The therapeutic monoclonal antibodies, cetuximab (Erbitux) and blinatumomab (Blincyto), were purchased from the Food and Drug Administration (FDA)-designated pharmacy (WEP Clinical, Morrisville, MC). The EGF, recombinant CD3, and recombinant EGFR were obtained from RayBiotech (Peachtree Corners, GA, USA). The control bispecific antibodies anti-bGal-hCD3 (cat# bimab-bgalhcd3) and anti-hCD19-bGal (cat# bimab-hcd19bgal) were obtained from InvivoGen (San Diego, CA).
3
Comparison of TNBC Cell Lines and Therapeutic Antibodies
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The TNBC cell lines MDA-MB-231, BT-20 and MDA-MB-468 were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and propagated at 37 °C under 5% CO2 as described previously [21 (link),22 (link)]. Expi293F cells (cat# A14527 ThermoFisher Scientific, Waltham, MA, USA) are human kidney cells derived from 293F cell line and were maintained in the suspension culture using Expi293 Expression Medium. Therapeutic monoclonal antibodies cetuximab (Erbitux®) and atezolizumab (Tecentriq®) were purchased from the FDA-designated pharmacy. The EGF, recombinant PD-L1 and recombinant EGFR were obtained from RayBiotech (Peachtree Corners, GA, USA).
4
Profiling Angiogenic Factors in EC/VSMC
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Saphenous vein-derived ECs and VSMCs were separately cultured on 6-well plates and treated with TGFβ1 (0, 1, and 10 ng/ml) for 24 h. The resulting conditioned medium was harvested and used to determine the relative levels of AGFs by ELISA (VEGF-C, Ang-1, TGF-β1; R&D Systems Ltd) or Quantibody multiplex array (Angiogenin, Ang-2, EGF, bFGF, HB-EGF, HGF, Leptin, PDGF-BB, PlGF, VEGF-A; Raybiotech, Norcross, United States) according to the manufacturers’ instructions. Each experiment was performed on three separate occasions.
5
EGFR Ligand Quantification by ELISA
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Cells were seeded onto 6-well plates and incubated for 72 h before culture supernatants were collected for ELISA to determine ligand release. ELISA kits to detect the human 1 3
EGFR ligands EGF, HB-EGF, TGF-α, and amphiregulin were purchased from Ray Biotech, Inc..
EGFR ligands EGF, HB-EGF, TGF-α, and amphiregulin were purchased from Ray Biotech, Inc..
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