Mouse anti α sarcomeric actin

Manufactured by Merck Group

Mouse anti‐α sarcomeric actin is a laboratory reagent that can be used to detect the presence and distribution of α sarcomeric actin, a protein found in muscle cells. It is a monoclonal antibody that specifically binds to this target protein.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit anti gfp, by Abcam (1 mentions) Rabbit anti histone h3, by Merck Group (1 mentions) Odyssey imaging system, by LI COR (1 mentions) Any kd pre cast polyacrylamide gels, by Bio-Rad (1 mentions) Rabbit anti phospho histone h3 ser10, by Merck Group (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Protein block solution, by Agilent Technologies (1 mentions) Rabbit anti von willebrand factor, by Abcam (1 mentions) Rabbit anti ki67, by Abcam (1 mentions) Saponin, by Merck Group (1 mentions)

Spelling variants of this product

Anti-actin (1282 citations) Mouse anti-actin (311 citations) α-actin (101 citations) Anti-α-actin (34 citations) Mouse anti-α-actin (13 citations) α-sarcomeric actin (11 citations) Anti-α-sarcomeric actin (9 citations) Anti-sarcomeric actin (2 citations) α‐mouse actin (2 citations) Mouse anti- α-sarcomeric actin (α-SA) (2 citations)

3 protocols using mouse anti α sarcomeric actin

Quantifying Cardiomyocyte Histology

Check if the same lab product or an alternative is used in the 5 most similar protocols

For heart histology, all animals were killed at 6 months after cell injection (after echocardiography study), and excised hearts were cryosectioned (5 μm thickness). Heart cryosections were then fixed with 4% PFA, blocked/permeabilized and stained with rabbit anti‐GFP (Abcam) and mouse anti‐α sarcomeric actin (Sigma‐Aldrich) antibodies. Images were taken with a confocal microscope. To quantify myocyte signals, the images were split into difference fluorescent channels with the Image J software. The fluorescent intensity of the myocyte (alpha‐SA; red) channel was measured and normalized to the total intensity 14, 15.

Shen D., Shen M., Liang H., Tang J., Wang B., Liu C., Wang P., Dong J., Li L., Zhang J, & Caranasos T.G. (2018). Therapeutic benefits of CD90‐negative cardiac stromal cells in rats with a 30‐day chronic infarct. Journal of Cellular and Molecular Medicine, 22(3), 1984-1991.

+ Open protocol

+ Expand

Protein Extraction and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue was homogenized in 1× cell lysis buffer (CLB; Cell Signaling Technologies) with protease inhibitor cocktail (Roche) and 1 mM phenylmethylsulfonyl fluoride. The protein concentration was quantified using a BCA Protein Assay Kit (Pierce) and equal protein amounts were loaded and run on Any kD precast polyacrylamide gels (Bio-Rad), transferred to a polyvinylidene difluoride membrane, blocked with 5% non-fat dry milk or 3% bovine serum albumin (BSA) and probed overnight with primary antibody. Blots were washed and probed with horseradish peroxidase-conjugated secondary antibody, washed, incubated in enhanced chemiluminescence reagent and visualized with the Odyssey imaging system (LI-COR Biosciences). Alternatively, a chromogenic alkaline phosphatase system with one-step nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate on-membrane color development was used (Thermo Fisher Scientific). The antibodies used for blotting included rabbit anti-RBM20 (Novus; NBP1–91002), mouse anti-α-sarcomeric actin (Sigma–Aldrich; A2172), rabbit anti-phospho-histone H3 (Ser10) (Millipore; 06–570), rabbit anti-histone H3 (SCBT; sc-10809); mouse anti-sarcomeric myosin (DSHB; MF20) and mouse anti-FHL1 (SCBT; sc-374246).

Schneider J.W., Oommen S., Qureshi M.Y., Goetsch S.C., Pease D.R., Sundsbak R.S., Guo W., Sun M., Sun H., Kuroyanagi H., Webster D.A., Coutts A.W., Holst K.A., Edwards B.S., Newville N., Hathcock M.A., Melkamu T., Briganti F., Wei W., Romanelli M.G., Fahrenkrug S.C., Frantz D.E., Olson T.M., Steinmetz L.M., Carlson D.F, & Nelson T.J. (2020). Dysregulated ribonucleoprotein granules promote cardiomyopathy in RBM20 gene-edited pigs. Nature medicine, 26(11), 1788-1800.

+ Open protocol

+ Expand

Cardiac Immunohistochemistry: Detecting Vascular and Proliferative Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunohistochemistry, heart cryosections were fixed with 4% paraformaldehyde, permeabilized, and blocked with Protein Block Solution (Dako, Carpinteria, CA, http://www.dako.com) containing 1% saponin (Sigma-Aldrich) and then incubated with the following antibodies overnight at 4°C: rabbit anti–von Willebrand factor (Abcam, Cambridge, MA, http://www.abcam.com), mouse anti–α sarcomeric actin (Sigma-Aldrich), rabbit anti-Ki67 (Abcam), or fluorescein isothiocyanate (FITC)-conjugated isolectin B4 (Vector Laboratories, Burlingame, CA, http://vectorlabs.com). FITC- or Texas red secondary antibodies were used for detection. Images were taken with a confocal microscopy system.

Shen D., Tang J., Hensley M.T., Li T., Caranasos T.G., Zhang T., Zhang J, & Cheng K. (2016). Effects of Matrix Metalloproteinases on the Performance of Platelet Fibrin Gel Spiked With Cardiac Stem Cells in Heart Repair. Stem Cells Translational Medicine, 5(6), 793-803.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!