Crl 1711

Manufactured by American Type Culture Collection

CRL-1711 is a cell line derived from the lung tissue of a human female. It is a commonly used in vitro model for studying lung biology and disease.

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Lab products found in correlation

Igg2a, by Southern Biotech (1 mentions) Sf900ii medium, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Expi293f cells, by Thermo Fisher Scientific (1 mentions) Expi293 expression medium, by Thermo Fisher Scientific (1 mentions) Erlenmeyer shake flasks, by Corning (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Tnm fh, by Gemini Bio (1 mentions) Foetal calf serum, by Harvard Bioscience (1 mentions) Crl 1660, by American Type Culture Collection (1 mentions) Foetal calf serum, by Thermo Fisher Scientific (1 mentions)

4 protocols using crl 1711

Recombinant Baculovirus Production and VLP Generation

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Spodoptera frugiperda 9 (SF9) insect cells (CRL-1711; ATCC) were maintained in suspension in serum-free SF900-II medium (GIBCO-BRL) and used for production of recombinant baculoviruses (rBVs) and VLPs. RSV A2 and A2-K-line19F were used as described [19 (link)]. Monoclonal antibodies (mAb) D25 and 131-2A were purchased from Creative Biolobs and from Millipore. Pre-F protein with DS-Cav-1 mutations and post-F protein, and 5C4 mAb were generously provided from Dr. Graham (VRC, NIAID, NIH). Palivizumab mAb was kindly provided by Dr. Eun-Hyung Lee (Emory University). HRP conjugated anti-mouse antibody IgG, IgG1, IgG2a, and anti-human antibody IgG were purchased from Southern Biotech (Birmingham, AL).

Kwon Y.M., Lee Y., Kim K.H., Jung Y.J., Li Z., Jeeva S., Lee S., Moore M.L, & Kang S.M. (2019). Antigenicity and immunogenicity of unique prefusion-mimic F proteins presented on enveloped virus-like particles. Vaccine, 37(44), 6656-6664.

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Recombinant CCR5:[5P7]CCL5 Production

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Spodoptera frugiperda (Sf9) cells (ATCC, CRL-1711™) were used to produce recombinant CCR5:[5P7]CCL5. The cells were maintained in ESF 921 Protein-Free Insect Cell Culture Medium (Expression systems, 96-001-01) at 27°C with shaking at 140 rpm, and passaged every 72h.
Sf9 cells were obtained from and authenticated by ATCC. Sex of cells is not relevant.

Zheng Y., Han G.W., Abagyan R., Wu B., Stevens R.C., Cherezov V., Kufareva I, & Handel T.M. (2017). Structure of CC Chemokine Receptor 5 with a Potent Chemokine Antagonist Reveals Mechanisms of Chemokine Recognition and Molecular Mimicry by HIV. Immunity, 46(6), 1005-1017.e5.

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Baculovirus Rescue and Protein Expression

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Baculovirus rescue was performed in Sf9 cells (CRL-1711, ATCC). Sf9 cells were grown in Trichoplusia ni medium-formulation Hink insect cell medium (TNM-FH, Gemini Bioproducts) containing 10% fetal bovine serum (FBS; Sigma) and penicillin (100 U/mL)-streptomycin (100 μg/mL) solution (Gibco). BTI-TN-5B1-4 (High Five) cells used for protein expression were cultured in serum-free SFX medium (HyClone) containing penicillin (100 U/mL)-streptomycin (100 μg/mL) solution. Expi293F cells (ThermoFisher) used for protein expression were grown in Expi293 Expression Medium (Gibco) in 1 L Erlenmeyer shake flasks (Corning) at 37 °C and 125 RPM in a humidified incubator with 8% CO₂. Madin Darby canine kidney (MDCK) and human embryonic kidney (HEK) 293T cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS and penicillin (100 U/mL)-streptomycin (100 μg/mL) solution.

McMahon M., O’Dell G., Tan J., Sárközy A., Vadovics M., Carreño J.M., Puente-Massaguer E., Muramatsu H., Bajusz C., Rijnink W., Beattie M., Tam Y.K., Kirkpatrick Roubidoux E., Francisco I., Strohmeier S., Kanekiyo M., Graham B.S., Krammer F, & Pardi N. (2022). Assessment of a quadrivalent nucleoside-modified mRNA vaccine that protects against group 2 influenza viruses. Proceedings of the National Academy of Sciences of the United States of America, 119(45), e2206333119.

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Culturing Aedes, Spodoptera, and Acanthamoeba Cell Lines

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Aedes albopictus clone C6/36 larva cells (ATCC ® CRL-1660 ™ ) and Spodoptera frugiperda ovarian epithelial cells (Sf9) (ATCC ® CRL-1711 ™ ) were routinely maintained respectively at 28°C and 5%CO 2 in Dulbecco's modified essential medium (DMEM; Gibco Invitrogen, Basel, Switzerland) supplemented with 10% foetal calf serum (Biochrom, Berlin, Germany) or at 27°C in Grace insect medium (GIM; Gibco Invitrogen, Basel, Switzerland) supplemented with 10% foetal calf serum.
W. chondrophila strain WSU 86-1044 (ATCC VR-1470), E. lausannensis strain CRIB 30 and P. acanthamoebae strain Hall's coccus were grown at 32°C within Acanthamoeba castellanii strain ATCC 30010, as described in [19] .

Kebbi-Beghdadi C., Fatton M, & Greub G. (2015). Permissivity of insect cells to Waddlia chondrophila, Estrella lausannensis and Parachlamydia acanthamoebae. Microbes and infection, 17(11-12).

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