Anti nk1.1 fitc pk136

Manufactured by BD

Anti-NK1.1 FITC (PK136) is a fluorescein isothiocyanate (FITC)-conjugated monoclonal antibody that binds to the NK1.1 antigen, which is expressed on natural killer (NK) cells and some T cell subsets in mice. This antibody can be used for the identification and analysis of NK cells and NK T cells in flow cytometry applications.

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Lab products found in correlation

Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Anti cd19 fitc 1d3, by BD (1 mentions) Anti cd11b pecy7 m1 70, by BioLegend (1 mentions) Anti cd45 buv395 30 f11, by BD (1 mentions) Anti ly6g apc cy7 1a8, by BD (1 mentions) Streptavidin pe, by BD (1 mentions) Apc anti cd3 145 2c11, by BD (1 mentions) Rpmi medium, by Merck Group (1 mentions)

Spelling variants of this product

NK1.1 (PK136) (18 citations) Anti-NK1.1 (17 citations) Anti-NK1.1-FITC (7 citations) NK1.1/FITC (5 citations) Anti-NK1.1 (PK136) (4 citations)

2 protocols using anti nk1.1 fitc pk136

Comprehensive Myeloid Cell Immunophenotyping

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Fluorophore-conjugated antibodies against myeloid lineage markers (CD11b, Ly6G, Ly6C, CD115), a TF IRF8 with IgG1 κ isotype control, other lineage markers (CD3ε, TCRγδ, B220, NK1.1, Ter119, CD19), and leukocyte common antigen CD45 were listed as below: LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Invitrogen, Thermo Fisher Scientific, catalog L34965), anti-CD3ε FITC (145-2C11) (eBioscience, Thermo Fisher Scientific, catalog 11-0031-85), anti-TCRγδ FITC (GL3) (eBioscience, Thermo Fisher Scientific, catalog 11-5711-82), anti-B220 FITC (RA3-6B2) (eBioscience, Thermo Fisher Scientific, catalog 11-0452-82), anti-NK1.1 FITC (PK136) (BD Biosciences, catalog 553164), anti-Ter119 FITC (TER-119) (BioLegend, catalog 116205), anti-CD19 FITC (1D3) (BD Biosciences, catalog 553785), anti-CD45 BUV395 (30-F11) (BD Biosciences, catalog 564279), anti-CD11b PE-Cy7 (M1/70) (BioLegend, catalog 101216), anti-Ly6G APC-Cy7 (1A8) (BD Biosciences, catalog 560600), anti-Ly6C PerCP-Cy5.5 (HK1.4) (eBioscience, Thermo Fisher Scientific, catalog 45-5932-82), anti-CD115 PE (AFS98) (eBioscience, Thermo Fisher Scientific, catalog 12-1152-81), anti-IRF8 APC (V3GYWCH) (eBioscience, Thermo Fisher Scientific, catalog 17-9852-80), and anti-IgG1 κ isotype control APC (P3.6.2.8.1) (eBioscience, Thermo Fisher Scientific, catalog 17-4714-82).

Chen T., Delano M.J., Chen K., Sperry J.L., Namas R.A., Lamparello A.J., Deng M., Conroy J., Moldawer L.L., Efron P.A., Loughran P., Seymour C., Angus D.C., Vodovotz Y., Chen W, & Billiar T.R. (2021). A road map from single-cell transcriptome to patient classification for the immune response to trauma. JCI Insight, 6(2), e145108.

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Characterizing Tumor-Infiltrating NK Cells

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C57BL/6 WT mice were inoculated intravenously with B16/BL6 (1.0 × 10⁵ cells/100 µl). After 17 d, colonies in the lung were carefully taken from the PBS-perfused lung under a stereo microscope and were incubated at 37°C for 1 h in RPMI medium (Sigma-Aldrich) containing DNase I (100 U/ml). The foci were mashed and passed through a mesh to prepare a single-cell suspension. Isolated cells, including tumor cells, were stained with biotinylated anti-mouse DNAM-1 followed by streptavidin-PE (BD Biosciences), anti-mouse TIGIT-PE (1G9; Tonbo), and anti-mouse CD96-PE (630612; R&D Systems) under staining with anti-CD45.2-PerCP-Cy5.5 (Ly-5.2; BD Biosciences), anti-CD3-APC (145-2C11; BD Biosciences), and anti-NK1.1-FITC (PK136; BD Bioscience) to gate on NK cells.

Okumura G., Iguchi-Manaka A., Murata R., Yamashita-Kanemaru Y., Shibuya A, & Shibuya K. (2020). Tumor-derived soluble CD155 inhibits DNAM-1–mediated antitumor activity of natural killer cells. The Journal of Experimental Medicine, 217(4), 1.

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