The following cultivation in YPD at 30 °C for 24 h, the cells were collected, washed twice with phosphate-buffered saline (PBS, pH 7.0), and suspended in PBS with 1 mg/mL bovine serum albumin (BSA) at a final OD600 of 1.0. Monoclonal mouse anti−V5−FITC antibody (Invitrogen−R96325) was added to the samples at 1:500 dilutions at 25 °C for 1 h. Cells were harvested and washed twice with PBS after immunostaining. The fluorescence distribution of 10,000 cells in each sample was analyzed using flow cytometry (CytoFLEX S, Beckman Coulter, Brea, CA, USA).
Monoclonal mouse anti v5 fitc antibody
Manufactured by Thermo Fisher Scientific
The Monoclonal mouse anti−V5−FITC antibody is a laboratory reagent used for the detection and identification of proteins tagged with the V5 epitope. The antibody is conjugated with the fluorescent dye FITC, allowing for fluorescence-based detection and visualization of the target protein.
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2 protocols using monoclonal mouse anti v5 fitc antibody
1
V5-FITC Immunostaining of Yeast
2
Immunostaining and Flow Cytometry Analysis
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Cells were harvested and washed twice with phosphate buffered saline (PBS, pH 7.0), and suspended in PBS with 1 mg/mL bovine serum albumin (BSA). The cells were diluted to an OD600 of 1.0 and 300 μL of cell suspension was used as the sample for immunostaining. The monoclonal mouse anti-V5-FITC antibody (Invitrogen) was added to the samples at 1: 500 dilution at 25 °C for 1 h. The cells were harvested and washed twice with PBS after immunostaining. Flow cytometry analysis (FACS) used the FACSCanto II system (BD FACSCanto II, USA) and the CytoFLEX Platform (BECKMAN COULTER, USA).
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