PC3 cells were seeded directly on coverslips in 6 wells plates at a density of 1 × 105cells/well and allowed to grow until 70-80% confluence. Cells were then treated with 100 nM Triptolide or DMSO for 24h and SA-β-Galactosidase activity was using the SA-β-Galactosidase staining kit (Beyotime) according to the manufacturer instructions. Senescent cells stain in blue. Image was recovered using a CKX41 inverted microscope fitted with a DP22 microscope digital camera (Olympus).
Dp22 microscope digital camera
Manufactured by Olympus
Sourced in Japan
The DP22 is a digital camera designed for use with Olympus microscopes. It features a high-resolution sensor and is capable of capturing detailed images of microscopic samples. The DP22 is intended to provide users with a reliable and efficient tool for imaging and documentation purposes in various laboratory and research settings.
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3 protocols using dp22 microscope digital camera
1
Senescence Evaluation in PC3 Cells
2
WST-8 Assay for Cell Viability
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For determination of cell viability, a WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) assay using Cell Count Reagent SF (Nacalai Tesque) was performed. In brief, B16BL6 cells (500 cells/well) were seeded in 96-well plates, allowed to adhere overnight, and cultured in the absence or presence of D4-2 in RPMI 1640 supplemented with 10% FBS and 0.1% DMSO for 1 to 3 days. The cells were then incubated with the WST-8 labeling mixture (Cell Count Reagent SF) for 3 h before measurement of absorbance at 450 nm with a microplate reader (2030 ARVO X4).
Cell Morphology Assay B16BL6 cells (1 3 10 5 ) were seeded in a 35-mm dish, incubated overnight, and then exposed for 48 h to either vehicle (0.1% DMSO) or D4-2 (1 or 10 mM) in RPMI 1640 medium supplemented with 10% FBS. Cell morphology was observed with a CKX53 microscope (Olympus, Tokyo, Japan), and images were captured with a DP22 microscope digital camera (Olympus).
Cell Morphology Assay B16BL6 cells (1 3 10 5 ) were seeded in a 35-mm dish, incubated overnight, and then exposed for 48 h to either vehicle (0.1% DMSO) or D4-2 (1 or 10 mM) in RPMI 1640 medium supplemented with 10% FBS. Cell morphology was observed with a CKX53 microscope (Olympus, Tokyo, Japan), and images were captured with a DP22 microscope digital camera (Olympus).
3
Quantification of B. burgdorferi Morphology
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