PBMCs were isolated by Ficoll-Paque centrifugation (as described above). Total B-cells were isolated by using the Human B-Cell Isolation Kit II, (Miltenyi Biotec, Bergisch Gladbach, Germany), from fresh PBMCs. The purity of B-cells was verified by flow cytometry using the monoclonal anti-human CD19 fluorescein isothiocyanate (FITC). The percentage of memory B-cells was assessed by flow cytometry using monoclonal anti-human CD19 fluorescein, anti-human CD27 allophycocyanin and anti-human CD38 Pacific Blue antibodies (BD Biosciences, San Jose, CA, USA). The majority of B-cells (>80%) were memory cells expressing CD19/CD27 and low CD38 [29 (link)].
Monoclonal anti human cd19 fluorescein
Manufactured by BD
Sourced in United States
Monoclonal anti-human CD19 fluorescein is a laboratory reagent used for the detection and identification of CD19-positive cells in biological samples. It consists of a monoclonal antibody that specifically binds to the CD19 cell surface antigen, which is expressed on B lymphocytes. The antibody is conjugated with the fluorescent dye fluorescein, allowing the labeled cells to be detected and analyzed using flow cytometry or other fluorescence-based methods.
Automatically generated - may contain errors
2 protocols using monoclonal anti human cd19 fluorescein
1
Isolation and Characterization of Memory B-Cells
2
Isolation and Characterization of Human Memory B-Cells
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Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque centrifugation (GE Healthcare, MA, USA). Total B-cells were isolated by using the Human B-Cell Isolation Kit II (Miltenyi Biotec, Bergisch Gladbach, Germany), from fresh PBMCs. The purity of B-cells was verified by flow cytometry using monoclonal anti-human CD19 fluorescein isothiocyanate (FITC). The percentage of memory B-cells was assessed by flow cytometry using monoclonal anti-human CD19 fluorescein, anti-human CD27 allophycocyanin and anti-human CD38 Pacific Blue antibodies (BD Biosciences, San Jose, CA, USA). The majority of B-cells were memory cells expressing CD19/CD27 and low CD38 [19 ,20 (link)].
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