Hepatic triglyceride secretion assays were performed at the University of Michigan Mouse Metabolic Phenotyping Center as previously described (Millar et al., 2005 (link)). Blood levels of glucose were measured using a glucometer (Acucheck, Roche, Basel Switzerland) and plasma levels of cholesterol (SB-1010–225, Fisher Scientific, Hampton NH) and triglycerides (10010303, Cayman Chemical, Ann Arbor MI) were determined using colorimetric assay kits. Plasma APOB levels were determined by ELISA (ab230932, abcam, Cambridge UK).
Sb 1010 225
Manufactured by Thermo Fisher Scientific
Sourced in Switzerland, United Kingdom
The SB-1010–225 is a laboratory mixing device. It provides gentle, constant agitation for various applications.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using sb 1010 225
1
Hepatic Triglyceride Secretion and Blood Lipid Profiling
2
Colorimetric Cholesterol Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total cholesterol in sera was determined using a colorimetric assay (SB-1010–225, Fisher Scientific, Hampton NH) according to the manufacturer’s instructions.
3
Colorimetric Cholesterol Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total cholesterol in sera was determined using a colorimetric assay (SB-1010–225, Fisher Scientific, Hampton NH) according to the manufacturer’s instructions.
4
Multiparametric Serum Lipoprotein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sera were analyzed by a colorimetric assay for total cholesterol (SB-1010–225, Fisher Scientific, Hampton NH) and by ELISAs for PCSK9 (MPC900, R&D Systems, Minneapolis MN) and APOB (ab230932, abcam, Cambridge UK). Serum lipoprotein fractionation assays were performed at the University of Cincinnati Mouse Metabolic Phenotyping Center. Sera were pooled from 5 mice for each genotype and fractionated by fast liquid protein chromatography (FPLC) into 50 fractions. Cholesterol (NC9343696, Fisher, Hampton NH) and triglyceride (TR213, Randox Laboratories, Crumlin UK) content in each fraction were determined using a microliter plate enzyme-based assay. Liver function tests were performed at the University of Michigan In-Vivo Animal Core (IVAC) with sera collected from individual mice using a Liasys analyzer (AMS Alliance).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!