In vivo stability was measured by taking blood sample before and after i.v. injection at 1, 5, 10 and every 10 min, where time 0 min corresponds to injection time. Serum samples were obtained by spinning down blood samples for 5 min, then diluted 100 times for analysis. BBB transport was measure by collecting dialysate from microdialysis at flow rate of 0.5 μl/min. Dialysate samples were collected during every 10 min before and after injection. Both serum and dialysate samples were stored on dry ice or −80°C. Samples were ZipTip® cleaned before being quantified by nano liquid chromatography-tandem mass spectrometry. Self-packed 10∼15 cm analytical column (ID 75 μm, 3 μm particle, Reprosil-Pur 120 C18-AQ) and Thermo LTQ Orbitrap Velos were used for analysis.
Pur 120 c18 aq
Manufactured by Thermo Fisher Scientific
The Pur 120 C18-AQ is a reversed-phase chromatography column designed for the separation of a wide range of analytes. It features a C18 bonded stationary phase and is suitable for use in aqueous mobile phase conditions.
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Lab products found in correlation
2 protocols using pur 120 c18 aq
1
In vivo Pharmacokinetic Evaluation
2
In vivo Pharmacokinetic Evaluation
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In vivo stability was measured by taking blood sample before and after i.v. injection at 1, 5, 10 and every 10 min, where time 0 min corresponds to injection time. Serum samples were obtained by spinning down blood samples for 5 min, then diluted 100 times for analysis. BBB transport was measure by collecting dialysate from microdialysis at flow rate of 0.5 μl/min. Dialysate samples were collected during every 10 min before and after injection. Both serum and dialysate samples were stored on dry ice or −80°C. Samples were ZipTip® cleaned before being quantified by nano liquid chromatography-tandem mass spectrometry. Self-packed 10∼15 cm analytical column (ID 75 μm, 3 μm particle, Reprosil-Pur 120 C18-AQ) and Thermo LTQ Orbitrap Velos were used for analysis.
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