The chemicals and reagents used for the synthesis of haptens were of analytical grade and were purchased from Sigma (St. Louis, MO) or Thermo Fisher Scientific (Rockford, IL). Bovine serum albumin (BSA), Ovalbumin (OVA), Thyroglobulin (Thy), 3,3’, 5,5’-tetramethylbenzidine (TMB), luminol and 4-iodophenol (PIP) were purchased from Sigma (St. Louis, MO). Goat anti-rabbit IgG-horseradish peroxidase was supplied by Abcam (Cambridge, MA). Standards (dicamba, 1 , and its analogs, 5-hydroxydicamba, 2 ; 2,3,5-trichlorobenzoic acid, 3 ; 2,3,6-trichlorobenzoic acid, 4 ; clopyralid, 5 ; picloram, 6 ; chloramben, 7 and chlorfenac, 8 ) (Figure 1 )) were purchased from Sigma (St. Louis, MO), Thermo Fisher Scientific (Rockford, IL) or Chem Service, Inc. (West Chester, PA). OriginPro 8.1 (OriginLab, Northampton, MA) was used for processing of the analytical data.
Chloramben
Manufactured by Merck Group
Chloramben is a laboratory chemical compound used in various analytical and research applications. It is a white crystalline solid with a molar mass of 174.59 g/mol. Chloramben serves as a reagent or analytical standard in various laboratory procedures, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.
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2 protocols using chloramben
1
Hapten Synthesis and Analytical Characterization
2
Chloramben-Carboxylic Acid Purification
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Chloramben (95% purity) was supplied by Sigma-Aldrich and used as received. Carboxylic acids were of analytical grade pur chased from Panreac. The background electrolytes, Na 2 SO 4 and NaCl, and FeSO 4 •7H 2 O used as catalyst source were of analytical grade from Fluka, Merck and Sigma-Aldrich, respectively. Other chemicals were either of analytical or HPLC grade supplied by Merck and Panreac. The synthetic solutions were prepared in ultrapure water (resistivity > 18.2 MΩ cm) from a Millipore Milli-Q system.
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