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Anti il 6 antibody

Manufactured by BD
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Sourced in Denmark, United Kingdom

The Anti-IL-6 antibody is a laboratory reagent used to detect and quantify the presence of interleukin-6 (IL-6) in biological samples. IL-6 is a cytokine involved in the inflammatory response and immune system regulation. The antibody can be used in various immunoassay techniques to measure IL-6 levels in research and diagnostic applications.

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Lab products found in correlation

Hrp conjugated streptavidin, by BD (1 mentions) Maxisorp 96 well plate, by Thermo Fisher Scientific (1 mentions) Nsc74859, by Selleck Chemicals (1 mentions) Sgp130fc, by R&D Systems (1 mentions) Cy3 conjugated secondary antibody, by Agilent Technologies (1 mentions) Anti mac3, by BD (1 mentions) Neomarker, by Thermo Fisher Scientific (1 mentions) Anti tgf β1 antibody, by Abcam (1 mentions)

Spelling variants of this product

Anti-IL-6 (7 citations) Rat anti-human IL-6 capture antibody (5 citations) Biotinylated rat anti-human IL-6 detection antibody (3 citations) Biotinylated anti-mouse IL-6 antibody (2 citations) Rat anti-mouse IL-6 capture antibody (2 citations) Rat anti-human IL-6 detection antibody (2 citations)

3 protocols using anti il 6 antibody

1

IL-6 Quantification by ELISA

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To measure the level of IL-6, ELISA was performed. Briefly, MaxiSorp 96-well plates (Nunc, Roskilde, Denmark) were coated with antigen or anti-IL-6 antibody (BD Biosciences, Franklin Lakes, NJ, USA). After adding each sample or recombinant standard protein, they were detected with biotin-conjugated anti-IL-6 antibody followed by HRP-conjugated streptavidin (BD Biosciences). Finally, substrate solution was added, and color development was measured using an ELISA reader (Packard Instrument, Meriden, CT, USA). Antibody titers were expressed as the reciprocal log2 titer of the highest sample dilution that gave an OD430 of 0.08, the value of the PBS blank.
Kim S.H., Lee H.Y, & Jang Y.S. (2015). Expression of the ATP-gated P2X7 Receptor on M Cells and Its Modulating Role in the Mucosal Immune Environment. Immune Network, 15(1), 44-49.
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2

Blocking IL-6 Signaling Pathway

Cited 1 time
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To block the signaling pathway, IL-6 was pre-incubated with 10 μg/ml of anti-IL-6 antibody (BD Biosciences) at 37°C in culture media for 30 min before the media was used to treat cells. Soluble gp130 Fc (sgp130Fc; 10 μg/ml; R & D Systems) was directly added into the IL-6-containing media for the treatment. Cells were pre-treated with NSC74859 (100 μM; Selleckchem) for 1 hr at 37°C before the addition of IL-6 and fresh NSC74859. Cells were then subjected to ICC and/or total lysate fractionation to examine hMSH3 localization.
The construct expressing constitutively activated STAT3 (S3c) tagged with FLAG (S3c-FLAG) was a kind gift from Dr. Beverly Barton (UMDNJ, Newark, NY) (24 (link)). Forty-eight hours post-transfection, cells were harvested to prepare cell lysates for WB or subsequent total cell lysate fractionation. A fraction of transfected cells were seeded onto 4-well chamber slides. Cells on the slides were fixed 48-hr post transfection for IFM studies.
Tseng-Rogenski S., Hamaya Y., Choi D.Y, & Carethers J.M. (2014). Interleukin 6 Alters Localization of hMSH3, Leading to DNA Mismatch Repair Defects in Colorectal Cancer Cells. Gastroenterology, 148(3), 579-589.
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3

Immunofluorescence Staining of Mouse Tissues

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Three sections per mouse (n = 5–6) were stained using anti- TGF-β1 antibody (Abcam, Cambridge, UK), anti-IL-6 antibody or anti-Mac3 (BD Pharmingen, San Jose, CA, USA) followed by fluorescein isothiocyanate (FITC)-conjugated secondary antibody. Double staining was performed using anti-smooth muscle actin (SMA, DAKO, Hamburg, Germany) or anti-MPO antibody (Neomarkers, Thermo Fisher Scientifics, Schwerte, Germany), followed by Cy3-conjugated secondary antibody (DAKO, Hamburg, Germany). The images were made using DISKUS (Hilgers, Königswinter, Germany). The contrast amplification and overlay were performed using DISKUS (Hilgers, Königswinter, Germany).
Curaj A., Schumacher D., Rusu M., Staudt M., Li X., Simsekyilmaz S., Jankowski V., Jankowski J., Dumitraşcu A.R., Hausenloy D.J., Schuh A, & Liehn E.A. (2020). Neutrophils Modulate Fibroblast Function and Promote Healing and Scar Formation after Murine Myocardial Infarction. International Journal of Molecular Sciences, 21(10), 3685.
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