The measurement uncertainties of the diameter and concentration as determined by RPS, NTA and FCM in Figs. 1 –3 were based on measurements of traceable reference beads (Nanosphere, Thermo Fisher, Waltham, MA) as described previously (16 (link)). The error bars represented the average deviation of the measured mean diameter and concentration of the reference beads from their expected values. Please see Supplementary File S2 and Table S2 for detailed information.
Nanosphere
Manufactured by Thermo Fisher Scientific
The Nanosphere is a versatile lab equipment designed for the analysis and characterization of nanoparticles. It utilizes light scattering principles to measure the size, size distribution, and concentration of nanoparticles in liquid samples. The Nanosphere is capable of analyzing a wide range of nanoparticle materials with sizes ranging from 1 to 1000 nanometers.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nanosphere
1
Measurement Uncertainties in Particle Analysis
2
Nanoparticle Tracking in Artificial Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To mimic the
physiological conditions of human blood, an artificial blood medium
was formulated by adjusting the viscosity of the EGM with the addition
of xanthan gum according to the literature.34 (link) A concentration of 0.066 g/L was used to obtain a viscosity of 4
× 10–3 Pa s and a shear rate of 200 s–1. Polystyrene NPs (200 nm Nanosphere, Thermofisher) were added into
the blood analogue at a typical (v/v) ratio of 1:5000 to avoid interparticle
hydrodynamic interactions ensuring single NP tracking.
physiological conditions of human blood, an artificial blood medium
was formulated by adjusting the viscosity of the EGM with the addition
of xanthan gum according to the literature.34 (link) A concentration of 0.066 g/L was used to obtain a viscosity of 4
× 10–3 Pa s and a shear rate of 200 s–1. Polystyrene NPs (200 nm Nanosphere, Thermofisher) were added into
the blood analogue at a typical (v/v) ratio of 1:5000 to avoid interparticle
hydrodynamic interactions ensuring single NP tracking.
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