Goat anti rabbit secondary

Manufactured by Thermo Fisher Scientific

The Goat anti-rabbit secondary is a laboratory reagent used in various immunological techniques. It is a secondary antibody that specifically binds to rabbit primary antibodies, enabling the detection and visualization of target molecules in biological samples.

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Lab products found in correlation

Anti cd45 clone 30 f11, by BioLegend (1 mentions) Zombie aqua viability dye, by BioLegend (1 mentions) Mojosort mouse anti apc nanobeads, by BioLegend (1 mentions) Anti epcam clone g8.8, by BioLegend (1 mentions) Anti epcam, by BioLegend (1 mentions) Nupage bis tris gel, by Thermo Fisher Scientific (1 mentions) Protease phosphatase inhibitor cocktail, by Cell Signaling Technology (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)

2 protocols using goat anti rabbit secondary

Flow Cytometry Analysis of Intestinal Epithelial Cells and Rotavirus Infection

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Dissociated cells were collected and stained for flow cytometry. Cells were stained with Zombie Aqua viability dye (BioLegend), Fc receptor-blocking antibody (CD16/CD32; BioLegend), anti-EpCAM (clone G8.8; BioLegend), and anti-CD45 (clone 30-F11; BioLegend). For analysis of murine rotavirus infection, cells were stained with anti-rotavirus (polyclonal; ThermoFisher, #PA1-7241) followed by goat anti-rabbit secondary (ThermoFisher). All data were analyzed using FlowJo software (BD Biosciences). Gates were set based on unstained and single-fluorophore stains. IECs were selected by gating on live, EpCAM-positive, CD45-negative cells. Gates for murine rotavirus infection were set based on naïve samples.
Where indicated, dissociated cells were enriched using MojoSort Mouse anti-APC Nanobeads (BioLegend, #480072) after flow cytometry staining for anti-EpCAM and anti-CD45 with APC fluorophores by following manufacturer protocols.

Van Winkle J.A., Peterson S.T., Kennedy E.A., Wheadon M.J., Ingle H., Desai C., Rodgers R., Constant D.A., Wright A.P., Li L., Artyomov M.N., Lee S., Baldridge M.T, & Nice T.J. (2022). Homeostatic interferon-lambda response to bacterial microbiota stimulates preemptive antiviral defense within discrete pockets of intestinal epithelium. eLife, 11, e74072.

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Immunoblotting for Phosphorylated ERK

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Control or ivermectin injected samples were harvested and placed directly into radioimmunoprecipitation assay buffer (RIPA buffer) containing a protease/phosphatase inhibitor cocktail (Cell Signaling Technology). The tissue was homogenized using the pestle mortar mixer (Argos Technologies), centrifuged and the supernatant was placed into a new tube. The protein concentration was determined using absorbance at 280 nm. Samples were separated on a 4–12% Bis-Tris NuPAGE gel (Life Technologies) and transferred onto a nitrocellulose membrane. Membranes were probed with anti-Erk or anti-phospho-Erk antibodies (Cell Signaling Technologies) overnight at 4 °C. After washing, membranes were incubated with goat anti-rabbit secondary (Thermo Scientific) and developed using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific).

Sabin K., Santos-Ferreira T., Essig J., Rudasill S, & Echeverri K. (2015). Dynamic membrane depolarization is an early regulator of ependymoglial cell response to spinal cord injury in axolotl. Developmental biology, 408(1), 14-25.

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