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Primeflow assay

Manufactured by Thermo Fisher Scientific
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The Primeflow assay is a flow cytometry-based tool designed for the detection and quantification of multiple mRNA targets within single cells. It enables the simultaneous measurement of intracellular RNA and surface proteins in individual cells.

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Lab products found in correlation

Aria 3, by BD (1 mentions) Fortessa, by BD (1 mentions) Aria 2, by BD (1 mentions)

Close spelling variants of this product

PrimeFlow RNA assay PrimeFlow RNA Assay Kit PrimeFlow assay kit PrimeFlow

2 protocols using primeflow assay

1

Flow Cytometry Immune Cell Profiling

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Cells (0.5–5 106) were stained with appropriate antibodies at 4°C in the dark for 30-45 mins and were analyzed with a Fortessa (BD Biosciences) and FlowJo software (TreeStar). KCs, AMs, splenic macs, colonic macs and microglia were sorted using an ARIA II or ARIA III (BD, Biosciences). The full list of antibodies used can be found in the Key Resource Table. Primeflow assay (Thermo Fisher) for Zeb2 expression was performed in 96-well U bottom plates according to the manufacturer’s instructions using commercially available Zeb2 primers (Thermo Fisher).
Scott C.L., T’Jonck W., Martens L., Todorov H., Sichien D., Soen B., Bonnardel J., De Prijck S., Vandamme N., Cannoodt R., Saelens W., Vanneste B., Toussaint W., De Bleser P., Takahashi N., Vandenabeele P., Henri S., Pridans C., Hume D.A., Lambrecht B.N., De Baetselier P., Milling S.W., Van Ginderachter J.A., Malissen B., Berx G., Beschin A., Saeys Y, & Guilliams M. (2018). The Transcription Factor ZEB2 Is Required to Maintain the Tissue-Specific Identities of Macrophages. Immunity, 49(2), 312-325.e5.
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2

Characterizing CMV-CD19CAR T Cells

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After 14 days ex vivo expansion in the presence of IL-2 and IL-15, CMV-CD19CAR T cell products were analyzed with multicolor flow cytometry for the expression of immune receptors representing memory, activation and exhaustion. To analyze the clonal type of the products, we performed flow cytometry-based Vβ repertoire analysis using The IOTestBeta Mark TCR Vβ Repertoire Kit (representing ~70% of normal TCR Vβ repertoire) per the manufacture’s instruction. To simultaneously analyze protein and gene expression by flow cytometry, the PrimeFlow assay (Thermo Fisher) was performed per the manufacturer’s suggested protocol.
Wang X., Urak R., Walter M., Guan M., Han T., Vyas V., Chien S.H., Gittins B., Clark M.C., Mokhtari S., Cardoso A., Diamond D.J., Zaia J., Forman S.J, & Nakamura R. (2022). Large-scale manufacturing and characterization of CMV-CD19CAR T cells. Journal for Immunotherapy of Cancer, 10(1), e003461.
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