Hp innowax fsc column

The GC–FID analyses were carried out with capillary GC using an Agilent 6890N GC system (Agilent, Santa Clara, CA, USA), and the GC/MS analyses were performed on an Agilent 5975 GC–MSD system (Agilent, Santa Clara, CA, USA). An HP-Innowax FSC column (60 m × 0.25 mm, 0.25 μm film thickness, Agilent, Wilmington, DE, USA) was used for the analyses. The HPLC chromatographic separations were carried out using Shimadzu LC 20 System (Shimadzu, Tokyo, Japan). The mass spectra were recorded with AB Sciex 3200 Q TRAP mass spectrometer (AB Sciex, Toronto, Canada). GL Science Inertsil ODS 250 × 4.6 mm, 5 μm i.d. particle size, analytical column (GL Sciences, Tokyo, Japan) was used for the HPLC analyses. The turbidity of the standardized microbial sample solutions was measured using McFarland densitometer (Biosan McFarland Densitometer, Model Den-1B, Riga, Latvia). Antioxidant activity absorbances were recorded with a Biotek microplate reader (BioTek, Winooski, Vermont, USA). Chlorogenic acid, luteolin 7-glucoside, gallic acid, butylated hydroxytoluene (BHT), and L-ascorbic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA).

GC-MS analysis was examined by an Agilent 6890N GC and Agilent 5975 GC/MSD systems (Agilent Technologies, SEM Ltd., Istanbul, Turkey). HP-Innowax FSC column (60 m × 0.25 mm, 0.25 μm film thickness (Agilent Technologies) was used with a helium carrier gas at 0.8 mL/min as reported previously [24].

The Agilent 6890N GC system (Agilent 6890N GC, Santa Clara, CA, USA) was used. The simultaneous automatic injection was carried out using the same conditions in two identical columns (HP-Innowax FSC column (60 m × 0.25 mm, 0.25 μm film thickness), Agilent, Walt & Jennings Scientific, Wilmington, DE, USA) in the Agilent 5975 GC/MSD system. Relative percentages of the separated components were calculated using the FID chromatograms. For identification and characterization, in-house “Baser Library of Essential Oil Constituents” and MassFinder 3 Library (Wiley GC/MS Library, MassFinder Software 4.0) were deployed where authentic samples or the relative retention index (RRI) of n-alkanes was also considered [41 (link)].