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Axiophot 2 camera

Manufactured by Zeiss
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Sourced in Germany

The Axiophot 2 is a camera designed for microscopy applications. It captures high-quality digital images of samples observed through a microscope. The camera features a high-resolution sensor and advanced imaging capabilities to support various microscopy techniques.

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Lab products found in correlation

Isis imaging system, by MetaSystems (4 mentions) Spectrum orange, by Abbott (3 mentions) Spectrum green dutp, by Abbott (3 mentions) Axioplan 2 fluorescence microscope, by Zeiss (1 mentions)

4 protocols using axiophot 2 camera

1

Chromosome Analysis via FISH

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R- and G-banding chromosomal analysis and fluorescence in situ hybridization (FISH) analysis followed standard procedures. Probes used for FISH analysis are listed in Table S1. Non-commercial probes were labeled with SpectrumOrange- and SpectrumGreen-d-UTP (Abbott Molecular, Ottigne, Belgium) using random priming. FISH experiments were evaluated using an Axioplan 2 fluorescence microscope equipped with a charge-coupled device Axiophot 2 camera (Carl Zeiss Microscopy, Jena, Germany) and a MetaSystems Isis imaging system (MetaSystems, Altlussheim, Germany). Two to 10 abnormal metaphases and/or 200 interphase cells were evaluated in each FISH experiment.
Finalet Ferreiro J., Rouhigharabaei L., Urbankova H., van der Krogt J.A., Michaux L., Shetty S., Krenacs L., Tousseyn T., De Paepe P., Uyttebroeck A., Verhoef G., Taghon T., Vandenberghe P., Cools J, & Wlodarska I. (2014). Integrative Genomic and Transcriptomic Analysis Identified Candidate Genes Implicated in the Pathogenesis of Hepatosplenic T-Cell Lymphoma. PLoS ONE, 9(7), e102977.
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2

Cytogenetic Analysis using G-banding and FISH

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G-banding chromosomal analysis and fluorescence in situ hybridization (FISH) followed routine methods. Probes applied for FISH are listed in Table S1. Non-commercial probes were labeled with SpectrumOrange- and SpectrumGreen-d-UTP (Abbott Molecular, Ottigne, Belgium) using random priming. FISH images were acquired with a fluorescence microscope equipped with an Axiophot 2 camera (Carl Zeiss Microscopy, Jena, Germany) and a MetaSystems ISIS imaging system (MetaSystems, Altlussheim, Germany). One to ten abnormal metaphases and/or 200 interphase cells were evaluated in each experiment.
Rouhigharabaei L., Finalet Ferreiro J., Tousseyn T., van der Krogt J.A., Put N., Haralambieva E., Graux C., Maes B., Vicente C., Vandenberghe P., Cools J, & Wlodarska I. (2014). Non-IG Aberrations of FOXP1 in B-Cell Malignancies Lead to an Aberrant Expression of N-Truncated Isoforms of FOXP1. PLoS ONE, 9(1), e85851.
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3

Cytogenetic and FISH Analysis Protocol

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Cytogenetic and fluorescence in situ hybridization (FISH) followed routine methods. Interphase FISH was performed on FFPE sections. FFPE sections were pretreated with SPOT-Light Tissue Pretreatment Kit (Life Techologies), following manufacturer’s protocol. Probes applied for FISH included LSI ALK, LSI MYC, LSI TP53/CEP17 (Abbott Molecular, Ottigne, Belgium or Rome, Italy) and home-brewed bacterial artificial chromosome (BAC) clones flanking TRAF1 or BRCA1 genes (Supplemental S2), selected from www.ensembl.org, or PRDM1 gene, kindly provided by Dr. Laura Pasqualucci (Columbia University, New York, NY, USA). Non-commercial probes were labeled with SpectrumOrange- and SpectrumGreen-d-UTP (Abbott Molecular) using random priming. FISH images were acquired with a fluorescence microscope equipped with an Axiophot 2 camera (Carl Zeiss Microscopy, Jena, Germany) and a MetaSystems ISIS imaging system (MetaSystems, Altlussheim, Germany). Approximately 100 interphase cells were evaluated in each analysis.
Abate F., Todaro M., van der Krogt J.A., Boi M., Landra I., Machiorlatti R., Tabbo’ F., Messana K., Barreca A., Novero D., Gaudiano M., Aliberti S., Di Giacomo F., Tousseyn T., Lasorsa E., Crescenzo R., Bessone L., Ficarra E., Acquaviva A., Rinaldi A., Ponzoni M., Longo D.L., Aime S., Cheng M., Ruggeri B., Piccaluga P.P., Pileri S., Tiacci E., Falini B., Pera-Gresely B., Cerchietti L., Iqbal J., Chan W.C., Shultz L.D., Kwee I., Piva R., Wlodarska I., Rabadan R., Bertoni F, & Inghirami G. (2014). A novel Patient Derived Tumorgraft model with TRAF1-ALK Anaplastic Large Cell Lymphoma translocation. Leukemia, 29(6), 1390-1401.
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4

Cytogenetic Analysis of Cancer Samples

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Conventional G-banding chromosomal analysis and FISH followed standard protocols. Karyotypes were described according to the International System for Human Cytogenetic Nomenclature (ISCN 2013) (Shaffer et al., 2013) . FISH probes used in this study, as well as their genomic localization, can be found in Table S1 and Table S2. Of note, the red-labelled probes included in the JAK2 break-apart (BA) assay cover PDL1 and PDL2 genes located downstream of JAK2 (Figure 1A). Noncommercial probes were directly labeled with SpectrumOrange-and SpectrumGreen-dUTP (Abbot Molecular, Ottigne, Belgium) using random prime reaction (Invitrogen, Carlsbad, USA). FISH experiments were evaluated using an Axioplan 2 fluorescence microscope equipped with a charge-coupled device Axiophot 2 camera (Carl Zeiss Microscopy, Jena, Germany) and a MetaSystems Isis imaging system (MetaSystems, Altlussheim, Germany). One to 10 abnormal metaphases (and facultatively 200 interphase cells) were evaluated in each FISH experiment. Interphase FISH analysis of cHL cases was previously described (Vandenberghe et al., 2015) .
Van Roosbroeck K., Ferreiro J.F., Tousseyn T., van der Krogt J.A., Michaux L., Pienkowska-Grela B., Theate I., De Paepe P., Dierickx D., Doyen C., Put N., Cools J., Vandenberghe P, & Wlodarska I. (2016). Genomic alterations of the JAK2 and PDL loci occur in a broad spectrum of lymphoid malignancies. Genes, chromosomes & cancer, 55(5).
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