Bodipy tmr cgp

Manufactured by Thermo Fisher Scientific

BODIPY-TMR-CGP is a fluorescent dye developed for use in biological research. It is a conjugate of the BODIPY fluorophore and the CGP compound, which is a cGMP-specific phosphodiesterase inhibitor. The dye can be used as a fluorescent label for various applications, including cellular imaging and in vitro binding assays.

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Lab products found in correlation

Propranolol, by Bio-Techne (2 mentions) Cell culture reagents, by Merck Group (2 mentions) Fetal calf serum (fcs), by GE Healthcare (2 mentions) Snap surface alexa fluor 488, by New England Biolabs (1 mentions) Cgp 20712a, by Bio-Techne (1 mentions) Snap surface 488, by New England Biolabs (1 mentions) Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions) Opti mem medium, by Thermo Fisher Scientific (1 mentions)

2 protocols using bodipy tmr cgp

Fluorescent Ligand Binding Assay

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Cell culture plastics were purchased from Fisher Scientific (Loughborough, UK) and cell culture reagents were from Sigma Aldrich (Gillingham, UK) except for fetal calf serum, which was obtained from PAA Laboratories (Pasching, Austria). SNAP-Surface™ Alexa Fluor ®488 was obtained from New England Biolabs (Ipswich, MA, USA). BODIPY-TMR-CGP was from Molecular Probes (Leiden, the Netherlands) and unlabelled CGP 12177, propranolol and CGP 20712A were from Tocris Cookson (Avonmouth, Bristol, UK). All other reagents were from Sigma Chemicals (Poole, UK).

Gherbi K., Briddon S.J, & Hill S.J. (2014). Detection of the secondary, low-affinity β1-adrenoceptor site in living cells using the fluorescent CGP 12177 derivative BODIPY-TMR-CGP. British Journal of Pharmacology, 171(23), 5431-5445.

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Cell Culture Reagents and Suppliers

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Cell culture plastics were purchased from Thermo Fisher Scientific (Loughborough, United Kingdom), and cell culture reagents were from Sigma-Aldrich (Gillingham, United Kingdom) except for fetal calf serum, which was obtained from PAA Laboratories (Pasching, Austria). Lipofectamine 2000 transfection reagent and Opti-MEM medium were from Invitrogen (Paisley, United Kingdom), and SNAP-Surface 488 was from New England Biolabs (Ipswich, MA, USA). BODIPY-TMR-CGP was from Molecular Probes (Leiden, The Netherlands), and unlabeled CGP 12177 and propranolol were from Tocris Cookson (Avonmouth, Bristol, United Kingdom). All other reagents were from Sigma Chemicals (Poole, United Kingdom).

Gherbi K., May L.T., Baker J.G., Briddon S.J, & Hill S.J. (2015). Negative cooperativity across β1-adrenoceptor homodimers provides insights into the nature of the secondary low-affinity CGP 12177 β1-adrenoceptor binding conformation. The FASEB Journal, 29(7), 2859-2871.

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