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Anti cd28 37

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The Anti-CD28 (37.51) is a laboratory reagent used for research purposes. It is an antibody that binds to the CD28 protein, which is expressed on the surface of T cells. The core function of this product is to facilitate the study of T cell activation and signaling pathways.

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Lab products found in correlation

Asparagine, by Merck Group (3 mentions) Fetal calf serum (fcs), by Merck Group (3 mentions) Non essential amino acids (neaa), by Merck Group (3 mentions) Dynabeads b220 depletion kit, by Thermo Fisher Scientific (3 mentions) Anti cd3 500a2, by Thermo Fisher Scientific (3 mentions) Khco3, by Merck Group (3 mentions) β mercaptoethanol, by Merck Group (3 mentions) Penicillin streptomycin, by Merck Group (3 mentions) L glutamine, by Merck Group (3 mentions) Sodium pyruvate, by Merck Group (3 mentions) Edta na2 2h2o 3, by Merck Group (2 mentions) Pharm lyse, by BD (2 mentions) Heparin, by Merck Group (1 mentions) 40 μm cell strainer, by Greiner (1 mentions) Complete dmem, by Merck Group (1 mentions) Nh4cl, by Merck Group (1 mentions)

Spelling variants of this product

Anti-CD28 (306 citations) Anti-mouse CD28 (clone 37.51, (7 citations) Soluble anti-CD28 (clone 37.51, (5 citations) Hamster anti-CD28 (37.51; (5 citations) Anti-CD28 antibody (37.51; (2 citations) Soluble anti-CD28 mAb (clone 37.51, (2 citations) Soluble anti-CD28 (37.51, (2 citations) Anti-CD28 antibody (clone 37.51, (2 citations) Anti-CD28 (37.51)-APC (2 citations) Anti-CD28 mAb (clone 37.51) (2 citations)

3 protocols using anti cd28 37

1

Isolation and Expansion of Lymphocytes

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Lymph nodes were passed through a 30-μm cell strainer (Sysmex, #04-004-2326) and spleens were passed through a 70-μm cell strainer (Greiner Bio-One, #542070), followed by gravity centrifugation (400 rcf, 4 °C, 10 min). Spleen samples underwent erythrocyte lysis with BD Pharm Lyse (BD Biosciences, #555899) or ACK solution (NH4Cl 8.024 mg/l (Merck, #1011451000), KHCO3 1.001 mg/l (Merck, #1048540500), EDTA.Na2·2H2O 3.722 mg/l (Merck, #1084181000)).
In retroviral transduction experiments, B cells were depleted from the cell suspensions using the Dynabeads B220 depletion kit (Thermo Fisher, #11441D). The remaining T cells were cultured in the presence of 0.5 μg/ml anti-CD3 (500A2, Invitrogen, #16-0033-86) and anti-CD28 (37.51, Invitrogen, #16-0281-86) stimulating antibodies in complete DMEM (Sigma-Aldrich, #D5671) (supplemented with 2 mM L-Glutamine (Sigma-Aldrich, #G7513), 100 μg/ml Penicillin/Streptomycin (Sigma-Aldrich, #4333), 36 mg/l Asparagine (Sigma-Aldrich, #A4284), 1 mM Sodium-Pyruvate (Sigma-Aldrich, #8636), 10 ml/l Non-essential amino acids (Sigma-Aldrich, #M7145), 4 μl/l β-Mercaptoethanol (Merck KGaA, #805740) and 10% fetal calf serum (Sigma-Aldrich, #S0615)).
Hiltensperger M., Beltrán E., Kant R., Tyystjärvi S., Lepennetier G., Moreno H.D., Bauer I.J., Grassmann S., Jarosch S., Schober K., Buchholz V.R., Kenet S., Gasperi C., Öllinger R., Rad R., Muschaweckh A., Sie C., Aly L., Knier B., Garg G., Afzali A.M., Gerdes L.A., Kümpfel T., Franzenburg S., Kawakami N., Hemmer B., Busch D.H., Misgeld T., Dornmair K, & Korn T. (2021). Skin and gut imprinted T helper cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity. Nature immunology, 22(7), 880-892.
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2

Isolation and Expansion of Lymphocytes

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Lymph nodes were passed through a 30-μm cell strainer (Sysmex, #04-004-2326) and spleens were passed through a 70-μm cell strainer (Greiner Bio-One, #542070), followed by gravity centrifugation (400 rcf, 4 °C, 10 min). Spleen samples underwent erythrocyte lysis with BD Pharm Lyse (BD Biosciences, #555899) or ACK solution (NH4Cl 8.024 mg/l (Merck, #1011451000), KHCO3 1.001 mg/l (Merck, #1048540500), EDTA.Na2·2H2O 3.722 mg/l (Merck, #1084181000)).
In retroviral transduction experiments, B cells were depleted from the cell suspensions using the Dynabeads B220 depletion kit (Thermo Fisher, #11441D). The remaining T cells were cultured in the presence of 0.5 μg/ml anti-CD3 (500A2, Invitrogen, #16-0033-86) and anti-CD28 (37.51, Invitrogen, #16-0281-86) stimulating antibodies in complete DMEM (Sigma-Aldrich, #D5671) (supplemented with 2 mM L-Glutamine (Sigma-Aldrich, #G7513), 100 μg/ml Penicillin/Streptomycin (Sigma-Aldrich, #4333), 36 mg/l Asparagine (Sigma-Aldrich, #A4284), 1 mM Sodium-Pyruvate (Sigma-Aldrich, #8636), 10 ml/l Non-essential amino acids (Sigma-Aldrich, #M7145), 4 μl/l β-Mercaptoethanol (Merck KGaA, #805740) and 10% fetal calf serum (Sigma-Aldrich, #S0615)).
Hiltensperger M., Beltrán E., Kant R., Tyystjärvi S., Lepennetier G., Moreno H.D., Bauer I.J., Grassmann S., Jarosch S., Schober K., Buchholz V.R., Kenet S., Gasperi C., Öllinger R., Rad R., Muschaweckh A., Sie C., Aly L., Knier B., Garg G., Afzali A.M., Gerdes L.A., Kümpfel T., Franzenburg S., Kawakami N., Hemmer B., Busch D.H., Misgeld T., Dornmair K, & Korn T. (2021). Skin and gut imprinted T helper cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity. Nature immunology, 22(7), 880-892.
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3

Murine T Cell Isolation and Transduction

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Lymph nodes and spleens were passed through a 40-μm cell strainer (Greiner Bio-One), followed by gravity centrifugation (400 g, 4 °C, 5 min). Blood was drawn from the heart into a syringe that had been flushed with heparin (5,000 U/mL) (Sigma-Aldrich) in PBS. Spleen and blood samples underwent erythrocyte lysis with ACK solution [NH4Cl 8.024 mg/l (Merck), KHCO3 1.001 mg/l (Merck), and EDTA.Na2·2H2O 3.722 mg/l (Merck)].
For retroviral T cell transduction experiments, B cells from lymph nodes and spleens were first depleted from the cell suspensions using the Dynabeads B220 depletion kit (Thermo Fisher). The remaining T cells were then cultured in the presence of 0.5 μg/mL anti-CD3 (500A2, Invitrogen) and anti-CD28 (37.51, Invitrogen)-stimulating antibodies in complete DMEM (Sigma-Aldrich) [supplemented with 2 mM L-Glutamine (Sigma-Aldrich), 100 μg/mL Penicillin/Streptomycin (Sigma-Aldrich), 36 mg/l Asparagine (Sigma-Aldrich), 1 mM Sodium-Pyruvate (Sigma-Aldrich), 10 mL/l Nonessential amino acids (Sigma-Aldrich), 4 μL/l β-Mercaptoethanol (Merck KGaA), and 10% fetal calf serum (Sigma-Aldrich)] at a concentration of 2 × 106 cells/ml in a 12-well tissue culture plate (2 mL/well) for 2 d.
Bauer I.J., Fang P., Lämmle K.F., Tyystjärvi S., Alterauge D., Baumjohann D., Yoon H., Korn T., Wekerle H, & Kawakami N. (2023). Visualizing the activation of encephalitogenic T cells in the ileal lamina propria by in vivo two-photon imaging. Proceedings of the National Academy of Sciences of the United States of America, 120(30), e2302697120.
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