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Rabbit anti g9a clonal c6h3

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Rabbit anti-G9a (clonal C6H3) is a primary antibody that recognizes the G9a protein, a histone methyltransferase enzyme involved in epigenetic regulation. The antibody is produced in rabbit and is specific to the C6H3 clone.

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Lab products found in correlation

Sybr green pcr kit, by Thermo Fisher Scientific (2 mentions) Magna chip kit, by Merck Group (2 mentions) Mouse monoclonal anti flag m2 f 3165, by Merck Group (2 mentions) Mouse anti g9a clonal, by R&D Systems (2 mentions) Mouse anti h3k9me2 ab1220, by Abcam (2 mentions)

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Anti-G9a (16 citations)

2 protocols using rabbit anti g9a clonal c6h3

1

Chromatin Immunoprecipitation (ChIP) Analysis

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ChIP analysis was performed using Magna ChIP kits (Millipore) according to the manufacturer’s instruction. The following antibodies were used in ChIP: mouse monoclonal anti-FLAG (M2) (F-3165, Sigma-Aldrich) (for FLAG-cyclin D1), mouse anti-G9a (clonal A8620A) (PP-A8620A-00) (R&D System), rabbit anti-G9a (clonal C6H3) (Cell signaling), and mouse anti-H3K9me2 (ab1220) (Abcam Inc.). For a negative control, mouse IgG was from the Magna ChIP kits (Millipore). SYBR Green PCR kit (Invitrogen) was used in the ChIP-real time PCR (ChIP-qPCR). The primers used in ChIP-qPCR are shown in the Supplemental Table 1.
Li Z., Jiao X., Di Sante G., Ertel A., Casimiro M.C., Wang M., Katiyar S., Ju X., Klopfenstein D.V., Tozeren A., Dampier W., Chepelev I., Jeltsch A, & Pestell R.G. (2019). Cyclin D1 integrates G9a-mediated histone methylation. Oncogene, 38(22), 4232-4249.
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2

Chromatin Immunoprecipitation (ChIP) Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP analysis was performed using Magna ChIP kits (Millipore) according to the manufacturer’s instruction. The following antibodies were used in ChIP: mouse monoclonal anti-FLAG (M2) (F-3165, Sigma-Aldrich) (for FLAG-cyclin D1), mouse anti-G9a (clonal A8620A) (PP-A8620A-00) (R&D System), rabbit anti-G9a (clonal C6H3) (Cell signaling), and mouse anti-H3K9me2 (ab1220) (Abcam Inc.). For a negative control, mouse IgG was from the Magna ChIP kits (Millipore). SYBR Green PCR kit (Invitrogen) was used in the ChIP-real time PCR (ChIP-qPCR). The primers used in ChIP-qPCR are shown in the Supplemental Table 1.
Li Z., Jiao X., Di Sante G., Ertel A., Casimiro M.C., Wang M., Katiyar S., Ju X., Klopfenstein D.V., Tozeren A., Dampier W., Chepelev I., Jeltsch A, & Pestell R.G. (2019). Cyclin D1 integrates G9a-mediated histone methylation. Oncogene, 38(22), 4232-4249.
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