The phosphofructokinase 1 (PFK1) (sc-31711) antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), while the phosphofructokinase 2 (PFK2) (bs-3528R), carnitine palmitoyltransferase-1A (CPT-1A) (bs-2047R), AMP-activated protein kinase (AMPK) (bs-1115R), β-actin (bs-10966R), phosphorylated PFK2 at Ser467 (bs-3331R), and phosphorylated pyruvate kinase M2 (p-PKM2) at Tyr105 (bs-3334R) antibodies were all purchased from Bioss Biotechnology (Beijing, China). The antibodies against PKM2 (15822–1-AP), hexokinase 2 (HK2) (22029–1-AP), α-ketoglutarate dehydrogenase (OGDH) (15212–1-AP), citrate synthase (CS) (16131–1-AP), isocitrate dehydrogenase 2 (IDH2) (15932–1-AP), lactate dehydrogenase B (LDHB) (14824–1-AP), and pyruvate dehydrogenase E1(PDHE1) (18068–1-AP) were all purchased from Proteintech Group (Chicago, IL, USA). The phosphorylated AMPK (Tyr172, 2531) antibody was acquired from Cell Signaling Technology (Beverly, MA, USA). The macrophage migration inhibitory factor (MIF) antibody was produced in our laboratory. Hematoxylin (AR11180–1) and eosin (AR11180–2) staining solutions were purchased from Boster Bioengineering Co. Ltd. (Wuhan, China), while the RIPA protein buffer and BCA protein assay kit (P0010) were procured from Beyotime Biotechnology (Shanghai, China). ECL-plus reagent (AR1111) was obtained from Bioss Biotechnology.
Hk2 22029 1 ap
Manufactured by Proteintech
Sourced in United States
HK2 (22029-1-AP) is a primary antibody targeted against the Hexokinase 2 (HK2) protein. Hexokinase 2 is an enzyme that catalyzes the phosphorylation of glucose to glucose-6-phosphate, a key step in glycolysis. This antibody can be used for applications such as western blotting, immunohistochemistry, and immunofluorescence to detect and study the HK2 protein.
Automatically generated - may contain errors
Lab products found in correlation
Bs 1115r, by Bioss Antibodies (1 mentions)
β actin bs 10966r, by Bioss Antibodies (1 mentions)
Ldhb 14824 1 ap, by Proteintech (1 mentions)
Bca protein assay kit p0010, by Beyotime (1 mentions)
Aldoa 11217 1 ap, by Proteintech (1 mentions)
Ab115730, by Abcam (1 mentions)
Anti p62 18420 1 ap, by Proteintech (1 mentions)
Bz x800, by Keyence (1 mentions)
Radioimmunoprecipitation lysis buffer, by Beyotime (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Bicinchoninic acid protein assay kit, by Beyotime (1 mentions)
4 protocols using hk2 22029 1 ap
1
Protein Expression Analysis in Metabolic Pathways
2
Western Blot Analysis of EMT Markers
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The specific primary antibodies against E-cadherin (ET1607–75), N-cadherin (ET1607–37), snail (ER1706–22), Flag (M1403–2), p-AKT(ET1607–73), AKT (ET1609–51), p-ERK (ET1610–13), ERK (ET1601–29), p-MEK (ET1609–50), CXCR4 (ER1802–28) and ENO1 (ET1705–56) were ordered from Hangzhou Hua-An Biotechnology Company. Other antibodies included β-actin (TA-09, ZSGB-BIO), MEK (sc-436, Santa Cruz), HK2 (22029–1-AP, Proteintech), ALDOA (11217–1-AP, Proteintech) and PGK1 antibody (sc-130335, Santa Cruz).
For serum PGK1 detection, the high abundance of serum albumin and IgG was deleted [21 (link), 22 (link)] using a reagent kit following procedures (Albumin and IgG Erasin Kit, C500063–0005, Shanghai Sangon Biotechnology). Reversible Ponceau staining was used as a loading control.
The density of target band in Western blot was measured with Image J software (NIH, USA) for semi-quantification of staining signals.
For serum PGK1 detection, the high abundance of serum albumin and IgG was deleted [21 (link), 22 (link)] using a reagent kit following procedures (Albumin and IgG Erasin Kit, C500063–0005, Shanghai Sangon Biotechnology). Reversible Ponceau staining was used as a loading control.
The density of target band in Western blot was measured with Image J software (NIH, USA) for semi-quantification of staining signals.
3
Immunohistochemical Analysis of Metabolic Markers
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Immunohistochemical (IHC) analyses were performed according to our previous study.2 (link) The sections were then incubated with primary antibodies anti-LC3 (#27751, 1:100, Signaling Technology), anti-p62 (18420-1-Ap, 1:100, Proteintech, Rosemont, Ill.), anti- hexokinase 2 (HK2;22029-1-Ap, 1:100, Proteintech), antiglucose transporter 1 (GLUT1;ab115730, 1:100, Abcam), and the same antibodies used in western blotting [ie, anti-PKM1 (1:25), PKM2 (1:200), and PTBP1 (1:100)]. The labeled sections were observed and captured using a fluorescent microscope (BZ-X800, Keyence, Osaka, Japan).
4
Quantifying Cellular Protein Expression
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Proteins present in cells and tissues were extracted utilizing the radioimmunoprecipitation lysis buffer (Beyotime). The quantification of protein concentration was accomplished using the bicinchoninic acid Protein Assay Kit (Beyotime). Subsequently, all cell lysates, each containing 40 μg protein, were subjected to SDS-PAGE and subsequently transferred onto polyvinylidene fluoride membranes through electrophoretic imprinting. The membrane was sealed with Tween-Tris buffered saline containing 5% skim milk at room temperature for a duration of 2 h. Subsequently, it was incubated with primary antibodies, namely GAPDH (2118, Cell Signaling Technology), PTMA (YN2871, Immunoway), hexokinase 2 (HK2; 22029-1-AP, Proteintech), and pyruvate kinases type M2 (PKM2; 4053, Cell Signaling Technology). Following this, the membrane containing protein bands was incubated with horseradish peroxidase-polymerized secondary antibody at room temperature for another 2 h. The imprints were visualized using the enhanced chemiluminescence detection system. The software FluorChem2.0 was employed to calculate the integral density values.
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