Trans astringin

Acetonitrile, sodium carbonate, hydrochloric acid, formic acid, ascorbic acid, gallic acid, Folin-Ciocalteu reagent, catechin, malvidine-3-O-glucoside, trans-piceid, trans-resveratrol, sodium phosphate dibasic dodecahydrate, potassium phosphate monobasic, sodium carbonate and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). Sodium acetate and trifluoroacetic acid were obtained from Carlo Erba Reagents (Peypin, France), and methanol and phloroglucinol from Biosolve Chimie (Dieuze, France). The solvents used were High Performance Liquid Chromatography (HPLC) grade. Deionized water was obtained from a Milli-Q Advantage A10 purification system from Millipore (Fontenay sous Bois, France). Alpha-glucosidase from Saccharomyces cerevisiae, acarbose and para-nitrophenyl alpha-D-glucopyranoside (p-NPG) were purchased from Analytic Lab (Castelnau-le-Lez, France). Trans-astringin and trans-piceatannol were obtained from Carbosynth (Compton, UK) and ChromaDex (Irvine, CA, USA), respectively. Hopeaphenol, isohopeaphenol, Ɛ-viniferin, δ-viniferin and ω-viniferin were isolated from a grapevine raw shoot. The cis-isomers were obtained using Ultraviolet-C (UV-C) irradiation (254 nm) from trans-isomers.

Folin Ciocalteu phenol reagent, sodium carbonate, 1,1-diphenyl-2-picrylhydrazyl (DPPH), Trolox, phloroglucinol, ascorbic acid, tartaric acid, sodium hydroxide, hydrochloric acid, lipopolysaccharide (LPS), Roswell Park Memorial Institute medium (RPMI) and Dulbecco’s Modified Eagle Medium (DMEM) mediums, fetal bovine serum (FBS), Griess reagent, 2′7′-dichlorodihydrofluoroscein diacetate acetyl (DCFH₂-DA), 3-(4,5-dimethylethiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), glutamine, gallic acid, catechin, malvidin-3-O-glucoside, trans-piceid and trans-resveratrol were obtained from Sigma Aldrich (Steinheim, Germany). Trans-astringin was purchased from Carbosynth (Berkshire, UK) and trans-piceatannol from ChromaDex (Los Angeles, CA, USA). Acetonitrile, methanol and water LC-MS were obtained from Biosolve (Dieuze, France) and trifluoroacetic acid and sodium acetate were purchased from Carlo Erba (Peypin, France). RAW 264.7 cells were provided by ATCC (Manassas, VA, USA). ε-viniferin, δ-viniferin, ω-viniferin, pallidol, parthenocissin A, miyabenol C, hopeaphenol and isohopeaphenol were isolated from a grapevine raw shoot in our laboratory. The cis-isomers stilbenes were obtained from trans-isomers using by applying Ultraviolet-C irradiation (254 nm).