Raji cells, a B lymphocyte cell line, were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum. Triptolide was purchased from Shanghai Yuanye Biotechnology Co., Ltd. (product number: B20709-20mg). The has-miR-146a inhibitor and transfection reagent were purchased from Guangzhou Ribo Biotechnology Co., Ltd.
Triptolide
Manufactured by Yuanye Bio-Technology
Sourced in China
Triptolide is a highly purified compound extracted from the Chinese herb Tripterygium wilfordii. It is a potent anti-inflammatory and immunosuppressive agent. Triptolide can be used as a research tool to study cellular processes and signaling pathways. Its core function is to inhibit the transcription of certain genes and modulate the activity of various proteins involved in immune and inflammatory responses.
Automatically generated - may contain errors
Lab products found in correlation
Ellagic acid, by Yuanye Bio-Technology (1 mentions)
Resveratrol, by Yuanye Bio-Technology (1 mentions)
P hydroxybenzoic acid, by Yuanye Bio-Technology (1 mentions)
P nitrophenyl α d glucopyranoside, by Merck Group (1 mentions)
Gallic acid, by Yuanye Bio-Technology (1 mentions)
α glucosidase, by Merck Group (1 mentions)
Acarbose, by Yuanye Bio-Technology (1 mentions)
Kaempferol, by Yuanye Bio-Technology (1 mentions)
Quercetin, by Yuanye Bio-Technology (1 mentions)
Formic acid, by Shanghai Lingfeng Chemical Reagent (1 mentions)
2 4 dinitrochlorobenzene, by Merck Group (1 mentions)
Methanol, by Shanghai Lingfeng Chemical Reagent (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Eca109, by BioVector (1 mentions)
Kyse410, by BioVector (1 mentions)
6 protocols using triptolide
1
Modulating miR-146a in Raji Cells
2
Pomegranate Peel Bioactive Compounds
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Pomegranate peel was purchased from Hebei Kang’an Biotech. Co. (Anguo, China). p-Nitrophenyl-α-D -glucopyranoside (pNPG) and α-glucosidase (100 U/mg) were obtained from Sigma-Aldrich (Shanghai, China). Acarbose, ellagic acid (EA), and the other compounds including nicotinic acid, isoguanosine, sclareol glycol, triptolide, sclareolide, p-hydroxybenzoic acid, kaempferol, gallic acid, quercetin, and resveratrol were purchased from Yuanye Biotech. Co. (Shanghai, China). All other chemicals used in this study were analytical grade.
3
Triptolide Bioavailability Enhancement Protocol
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Triptolide was purchased from Shanghai Yuanye Biological technology co., LTD (Shanghai, China). Triptolide standards were provided by Shanghai Ronghe Pharmaceutical Technology Co., Ltd. (Shanghai, China). 1,2-propanediol, methanol and formic acid were obtained from Shanghai Ling feng Chemical Reagent Co.(Shanghai, China). Capryol90 was provided by Gattefossé Co. (Lyon, France). OP-10 was purchased from Aladdin Industrial Inc. (Shanghai, China). 2,4-dinitrochlorobenzene was purchased from Sigma-Aldrich (St. Louis, USA). 4% paraformaldehyde solution was provided by Wuhan Google biological technology Co., Ltd. (Shanghai, China). Cytokine standard, IFN-γ, IL-4 and IL-8 ELISA kits were obtained from Bender Medsystems GmbH (South San Francisco, USA). Tripterygium wilfordii tablets were purchased from China resources san-jiu (Yellowstone) pharmaceutical co., Ltd. (Hubei, China). All other chemicals were of analytical purity and commercially available.
4
Culturing MDA-MB-231 Breast Cancer Cells
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Human breast cancer cell line MDA-MB-231 was maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Life Technologies) and 1% antibiotics (Gibco, Life Technologies). The cells were incubated at 37°C in a 5% CO2 humidified atmosphere until 75% confluent. Triptolide (≥98%; Yuanye Bio-Technology, Shanghai, P.R. China) was stored in a stock solution in dimethyl sulfoxide (DMSO; Sangon, Shanghai, P.R. China) at −20°C and diluted to various concentrations with serum-free culture medium when used.
5
Triptolide and LPS Treatment Protocol
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Triptolide was purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Product number: B20709, HPLC ≥98%). Lipopolysaccharides (LPS), from Escherichia coli 055:B5 (Product number: L2880) purchased from Sigma in the United States.
6
Triptolide Regulates circNOX4 in ESCC
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Human ESCC cells (KYSE410 and ECA109) and esophageal epithelial cell line (HET‐1A) were purchased from Biovector NTCC and grown in RPMI‐1640 medium (Hyclone) containing 10% fetal bovine serum (FBS: Hyclone) and 1% penicillin–streptomycin (Invitrogen) at 37°C with 5% CO2. Triptolide (Yuanye Bio‐Technology) was diluted to different concentrations (4, 8 and 12 nM) with DMSO solution (Sangon). When the cells reached 90% confluences, the cells were hatched with different concentrations of Triptolide for 24 h to detect circNOX4 expression. In subsequent functional tests, the cells were treated with 12 nM Triptolide for 24 h.
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