Log In Sign up
The largest database of trusted experimental protocols

Dmem 1 glutamax 1

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

DMEM (1×) Glutamax-I is a cell culture medium that supports the growth and maintenance of a variety of cell types. It contains L-glutamine, which is an essential amino acid for cell metabolism and proliferation.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Pyruvate, by Thermo Fisher Scientific (1 mentions) Hygromycin b, by Thermo Fisher Scientific (1 mentions) D glucose, by Thermo Fisher Scientific (1 mentions) Mem neaa, by Thermo Fisher Scientific (1 mentions) Dmem glutamax, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Fujifilm (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Hepes 1m, by Thermo Fisher Scientific (1 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (1 mentions) Dmem low glucose powder, by Merck Group (1 mentions) Acridine orange propidium iodide ao pi, by Logos Biosystems (1 mentions) Sodium bicarbonate 7.5 solution, by Thermo Fisher Scientific (1 mentions) D luciferin, by Cayman Chemical (1 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) D glucose, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

GlutaMAX (10236 citations) GlutaMAX-I (877 citations) DMEM GlutaMAX (797 citations) DMEM GlutaMAX-I (87 citations) DMEM+GlutamaxTM I medium (13 citations) DMEM/F12 + GlutaMAX-I™ (10 citations) DMEM-F12/1:1 + Glutamax (7 citations) DMEM (1X) +GlutaMAX™-I (5 citations) GIBCO® DMEM/F12 GlutaMAX™-I, (3 citations) DMEM plus Glutamax I (2 citations)

5 protocols using dmem 1 glutamax 1

1

Cell Culture and Transfection Protocols

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells—wild-type
(from ATCC, VA) and genetically modified—GFP-PEST HT1080 (generated
previously in our laboratory35 (link)), HEK293T
(from ATCC, VA), and SW480 (gift from Corinne Quittau-Prévostel,
from ATCC, VA) cells were grown in DMEM GlutaMAX (Gibco, IL) supplemented
with 10% FBS at 37 °C at 5% CO2. HT1080 cell medium
was further complemented with MEM NEAA (Gibco, IL). EGI-1 cells (gift
from Laura Fouassier and Nicolas Kuszla, from ATCC, VA) were grown
in DMEM 1× + GlutaMAX-1, 1 g/L d-glucose (“low
sucrose”) + pyruvate (21885-025, Gibco, IL) supplemented with
10% FBS, 100 UI/L of penicillin, 100 μg/mL of streptomycin,
and 10 mmol/L of HEPES. HeLa stably expressing NanoLuciferase-Hsp70
was generated in our laboratory,6 (link) as well
as DPH2KD HeLa cells35 (link) according to Picco
et al.36 (link) HeLa stably expressing CD8-GFP
was selected with hygromycin B (50 mg/mL, Invitrogen, MA) after lipofectamine
2000 transfection. Cells were transfected at a 70% confluency with
lipofectamine 2000 (Invitrogen, MA) according to the manufacturer’s
instructions.
Bui S., Dancourt J, & Lavieu G. (2023). Virus-Free Method to Control and Enhance Extracellular Vesicle Cargo Loading and Delivery. ACS Applied Bio Materials, 6(3), 1081-1091.
+ Open protocol
+ Expand
2

Overexpression of hnRNPK and PTBP1

Check if the same lab product or an alternative is used in the 5 most similar protocols
Clone vectors, hnRNPK in pCMV6-XL5, and PTBP1 in pCMV6-AC, were purchased from ORIGENE. After the cells were plated for 18 h, target protein clone vectors were transfected using Lipofectamine 2000 (Invitrogen). The transfected cells were maintained in DMEM (1×) + GlutaMAX-1 (Gibco) supplemented with 10% fetal bovine serum (Sigma) without penicillin-streptomycin (Wako) at 37°C, 5% CO2 for 6 h. Following this, pEGFP-C2 and SINEUP vectors were transfected into the cells as described above.
Toki N., Takahashi H., Sharma H., Valentine M.N., Rahman F.U., Zucchelli S., Gustincich S, & Carninci P. (2020). SINEUP long non-coding RNA acts via PTBP1 and HNRNPK to promote translational initiation assemblies. Nucleic Acids Research, 48(20), 11626-11644.
+ Open protocol
+ Expand
3

Comprehensive Cell Culture Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols
DMEM (1×) Glutamax-I, DPBS, penicillin-streptomycin (P/S), fetal bovine serum (FBS), 1× 0.05% trypsin-EDTA (T/E), B-27, HEPES (1 M), and sodium bicarbonate 7.5% solution were purchased from Gibco, Thermo Fisher Scientific (Waltham, USA). EA and D-luciferin were purchased from Cayman Chemical, Ann Arbor, USA, and PanReac AppliChem, Darmstadt, Germany, respectively. DMEM low-glucose powder, D-(+)-glucose, UA, dexamethasone (Dex), and dimethyl-sulfoxide (DMSO) were purchased from Sigma Aldrich, St. Louis, USA. Acridine Orange Propidium Iodide (AO-PI) was purchased from Logos Biosystems, Gyeonggi-do, South Korea.
Srimani S., Schmidt C.X., Gómez-Serranillos M.P., Oster H, & Divakar P.K. (2022). Modulation of Cellular Circadian Rhythms by Secondary Metabolites of Lichens. Frontiers in Cellular Neuroscience, 16, 907308.
+ Open protocol
+ Expand
4

Isolation and Expansion of Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
MSCs (n = 3) were isolated from bone marrow mononuclear cells from patients in the clinical improvement group of the FOCUS-CCTRN trial [54 (link)]. The cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) (1×)+GlutaMAX-I (Gibco, Carlsbad, CA, USA) supplemented with 10% of characterized fetal bovine serum of U.S. origin (HyClone, South Logan, UT, USA) and 1% of penicillin–streptomycin (Gibco). We expanded the cells under standard culture conditions (37 °C at 5% CO2) for over 4 passages and used approximately 2 × 106 cells to produce the EXOs. Bright-field images were acquired using a high-performance color CMOS C-mount microscope camera and AmScope 3.1 software (AmScope, Irvine, CA, USA).
Mesquita F.C., King M., da Costa Lopez P.L., Thevasagayampillai S., Gunaratne P.H, & Hochman-Mendez C. (2024). Laminin Alpha 2 Enhances the Protective Effect of Exosomes on Human iPSC-Derived Cardiomyocytes in an In Vitro Ischemia-Reoxygenation Model. International Journal of Molecular Sciences, 25(7), 3773.
+ Open protocol
+ Expand
5

Cultivation of HEK Cells in DMEM

Check if the same lab product or an alternative is used in the 5 most similar protocols
For HEK cell cultivation, DMEM (1×)+GlutaMAX™-I (Gibco Life Technologies, Carlsbad, CA, USA) containing 1% (v/v) Pen-Strep (Gibco Life Technologies) and 10% (v/v) of inactivated FCS (Gibco Life Technologies) was used. HEK cells were cultivated in 10 mm2 culture dishes at 37 °C and 5% CO2 humidified atmosphere.
Rimola V., Osthues T., Königs V., Geißlinger G, & Sisignano M. (2021). Oxaliplatin Causes Transient Changes in TRPM8 Channel Activity. International Journal of Molecular Sciences, 22(9), 4962.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!