Hydroxylapatite bio gel htp

Manufactured by Bio-Rad

Sourced in United States

Hydroxylapatite (Bio-gel HTP) is a chromatography media used for the purification and separation of biomolecules. It is a calcium phosphate-based adsorbent that can be used to separate proteins, nucleic acids, and other biological compounds based on their interactions with the hydroxylapatite surface.

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Lab products found in correlation

Celite 535, by Carl Roth (2 mentions) Amberlite bio beads sm 2, by Bio-Rad (2 mentions) Triton x 114, by Serva Electrophoresis (2 mentions) 14c 2 oxoglutarate, by PerkinElmer (2 mentions) 14c malate, by PerkinElmer (2 mentions) Asolectin, by Merck Group (2 mentions) N n methylenebisacrylamide, by Serva Electrophoresis (2 mentions) Acrylamide, by Serva Electrophoresis (2 mentions) Sodium dodecyl sulfate (sds), by Merck Group (1 mentions) Triton x 100, by Serva Electrophoresis (1 mentions) Hemin, by Merck Group (1 mentions) Triton x 100, by Bio-Rad (1 mentions) Cardiolipin, by Merck Group (1 mentions)

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Bio-Gel HTP Gel (2 citations)

2 protocols using hydroxylapatite bio gel htp

Isolation and Characterization of Mitochondria

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Hydroxylapatite (Bio-gel HTP) and Amberlite Bio-Beads SM-2 was obtained from Bio-Rad; Matrex Gel Orange was obtained from Amicon (Beverly, MA, USA); Triton X-100, Triton X-114, acrylamide, and N,N′-methylenebisacrylamide were obtained from Serva; egg-yolk phospholipids were obtained from Fluka; hemin, cardiolipin, 1,4-piperazine-diethanesulphonic acid (Pipes), Sodium dodecyl sulfate, (SDS) and asolectin were obtained from Sigma; celite 535 was obtained from Roth; Sephadex G-75 was obtained from Pharmacia; [¹⁴C] 2-oxoglutarate and [¹⁴C] malate were purchased from Perkin-Elmer Life Sciences (Waltham, MA, USA). All other chemicals used were of analytical grade.

Miniero D.V., Gambacorta N., Spagnoletta A., Tragni V., Loizzo S., Nicolotti O., Pierri C.L, & De Palma A. (2022). New Insights Regarding Hemin Inhibition of the Purified Rat Brain 2-Oxoglutarate Carrier and Relationships with Mitochondrial Dysfunction. Journal of Clinical Medicine, 11(24), 7519.

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Purification and Analysis of Mitochondrial Enzymes

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In regards to chemicals: [¹⁴C] 2-oxoglutarate and [¹⁴C] malate were purchased from Perkin-Elmer Life Sciences; hydroxylapatite (Bio-gel HTP) and Amberlite Bio-Beads SM-2 from Bio-Rad; Triton X-100, Triton X-114, acrylamide and N,N′-methylenebisacrylamide from Serva; egg yolk phospholipids from Fluka; Matrex Gel Orange from Amicon (Beverly, MA, USA); hemin, cardiolipin, 1,4-piperazine-diethanesulphonicacid (Pipes), sodium dodecyl sulfate (SDS) and asolectin from Sigma; celite 535 from Roth and Sephadex G-75 from Pharmacia. All other chemicals used were of analytical grade.

Miniero D.V., Spagnoletta A., Gambacorta N., Scalera V., Pierri C.L., Nicolotti O, & De Palma A. (2021). The Interaction of Hemin, a Porphyrin Derivative, with the Purified Rat Brain 2-Oxoglutarate Carrier. Biomolecules, 11(8), 1175.

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