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Abx574314

Manufactured by Abbexa

Abx574314 is a laboratory equipment product from Abbexa. It is designed for general laboratory use.

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3 protocols using abx574314

1

Plasma Hormone Levels Validation

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Plasma concentrations of LH, FSH, T4, P4, and E2 after validation25 (link) were evaluated using ELISA. LH and FSH concentrations were estimated using a kit for bovine (Cloud-Clone Corp., TX, USA, CEA441Bo for LH and CEA830Bo for FSH). The assay’s sensitivity for LH was 137.3 pg/ml and for FSH was 0.93 pg/ml. The mean intra- and inter- assays CVs were 10% and 12%, respectively. A commercial kit (Abbexa, abx574314) designed to measure the concentration of T4 has been assessed for measuring T4 in the plasma. The sensitivity of the assay was 1.95 pg/ml. The intra- and inter- assays CVs were 5.8 and 7.9%, respectively. According to the manufacturer’s instructions, the P4 and E2 concentrations were measured in plasma samples using an ELISA kit (Bioassay Technology Laboratory, Shanghai, China, E0018Bo for P4 and E0004Bo for E2). The assay’s sensitivities for P4 and E2 were 0.22 ng/ml and 2.53 pg/ml respectively. The mean intra- and inter -assays CVs were found to be 8% and 10%, respectively. Each run was proceeded in duplicate and further the average was considered as a single sample. All samples were detected in duplicate at 450 nm.
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2

Hormonal Profile Evaluation in Plasma

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Plasma concentrations of LH, FSH, T4, P4, and E2 after validation 25 were evaluated using ELISA. LH and FSH concentrations were estimated using a kit for bovine (Cloud-Clone Corp., TX, USA, CEA441Bo for LH and CEA830Bo for FSH). The assay's sensitivity for LH was 137.3 pg/ml and for FSH was 0.93 pg/ml.
The mean intra-and inter-assays CVs were 10% and 12%, respectively. A commercial kit (Abbexa, abx574314) designed to measure the concentration of T4 has been assessed for measuring T4 in the plasma. The sensitivity of the assay was 1.95 pg/ ml. The intra-and inter-assays CVs were 5.8 and 7.9%, respectively. According to the manufacturer's instructions, the P4 and E2 concentrations were measured in plasma samples using an ELISA kit (Bioassay Technology Laboratory, Shanghai, China, E0018Bo for P4 and E0004Bo for E2). The assay's sensitivities for P4 and E2 were 0.22 ng/ml and 2.53 pg/ml respectively. The mean intra-and inter -assays CVs were found to be 8% and 10%, respectively. Each run was proceeded in duplicate and further the average was considered as a single sample. All samples were detected in duplicate at 450 nm.
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3

Plasma Hormone Concentrations Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma concentrations of LH, FSH, T4, P4, and E2 after validation [25] (link) were evaluated using ELISA. LH and FSH concentrations were estimated using a kit for bovine (Cloud-Clone Corp., TX, USA, CEA441Bo for LH and CEA830Bo for FSH). The assay's sensitivity for LH was 137.3 pg/ml and for FSH was 0.93 pg/ml.
The mean intra-and inter-assays CVs were 10% and 12%, respectively. A commercial kit (Abbexa, abx574314) designed to measure the concentration of T4 has been assessed for measuring T4 in the plasma. The sensitivity of the assay was 1.95 pg/ ml. The intra-and inter-assays CVs were 5.8 and 7.9%, respectively. According to the manufacturer's instructions, the P4 and E2 concentrations were measured in plasma samples using an ELISA kit (Bioassay Technology Laboratory, Shanghai, China, E0018Bo for P4 and E0004Bo for E2). The assay's sensitivities for P4 and E2 were 0.22 ng/ml and 2.53 pg/ml respectively. The mean intra-and inter -assays CVs were found to be 8% and 10%, respectively. Each run was proceeded in duplicate and further the average was considered as a single sample. All samples were detected in duplicate at 450 nm.
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