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Sea563mu

Manufactured by Cloud-Clone
Sourced in China

The SEA563Mu is a laboratory equipment designed for specific functions. It is a compact and versatile device intended for use in controlled laboratory environments. The core function of this product is to perform tasks related to its intended purpose, without any further interpretation or extrapolation on its use.

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3 protocols using sea563mu

1

Cytokine Levels in Serum Quantification

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TNF-α (Cloud Clone Corp, SEA133Mu), IL-1β (Cloud clone crop, SEA563Mu), and IL-6 (Cloud Clone Corp, SEA079Mu) levels in serum were analyzed using a capture enzyme-linked immunoassay kit, in accordance with the manufacturer's instructions (Cloud Clone Corp).
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2

Serum Biomarker Measurement Protocol

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Blood specimens underwent a 10-min centrifugation at 3000 r/min for serum preparation. Serum tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), malondialdehyde (MDA) and kynurenic acid (KYNA) concentrations were assessed with specific mouse ELISAs (SEA133Mu, SEA563Mu, CED718Ge Cloud-Clone Corp, Wuhan, China, and E-EL-0060c, Elabscience, Wuhan, China, respectively) as directed by the manufacturers.
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3

Testicular Protein Profiling

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The testis was removed, washed with pre-cold PBS, cut into small pieces and placed in pre-cold lysis buffer (IS007, Cloud-Clone Corp., Wuhan, China) according to the instructions (1 ml lysis buffer supplemented with 50 mg tissue). Then, ultrasonic treatment was performed for clarification. After centrifuge (10 000g, 5 min), testicular tissue homogenization was obtained. Testicular tissue homogenization was used to detect the protein levels of IL-1β (SEA563Mu, Cloud-Clone Corp.), TNF-α (SEA133Mu, Cloud-Clone Corp.), and IL-10 (SEA056Mu, Cloud-Clone Corp.). The methods were determined with specific ELISA kits according to the manufacturer’s directions.
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