For immunoblotting, antibodies against STAT1 (#9172) and phospho-STAT1 (#9171) were acquired from Cell Signaling Biotechnology (Beverly, MA, USA). Anti-P-gp (Calbiochem, San Diego, CA, USA) and anti-actin (C4), -Klf4 (sc-166229), -HDAC4 (sc-5245) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-E-cadherin (ab15148), -N-cadherin (ab18203), -Vimentin (ab137321), -Bmi1 (ab126783), -Sox2 (ab97959), -Nanog (ab80892) and -Oct4 (ab109183) were obtained from Abcam (Cambridge, MA, USA).
Nanog ab80892
Manufactured by Abcam
Sourced in United States, United Kingdom
Nanog (ab80892) is a protein that plays a crucial role in the regulation of embryonic stem cell pluripotency and self-renewal. It is a member of the homeobox family of transcription factors and is considered a key marker of undifferentiated stem cells.
Automatically generated - may contain errors
Lab products found in correlation
Oct 4 ab19857, by Abcam (1 mentions)
β actin sc 47778, by Santa Cruz Biotechnology (1 mentions)
Creb 9197s, by Cell Signaling Technology (1 mentions)
Inverted confocal microscope, by Leica (1 mentions)
Nbp2 30332, by Novus Biologicals (1 mentions)
P akt1 2, by Cell Signaling Technology (1 mentions)
Mbs462020, by MyBioSource (1 mentions)
Imaris 7, by Leica (1 mentions)
3 protocols using nanog ab80892
1
Immunoblotting for Cell Signaling
2
Western Blot Protein Expression Analysis
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Detection of protein expression by western blot was carried out according to the established protocols described previously 14 (link). Western blot was performed using the following antibodies: SOX2 (sc-20088) and β-actin (sc-47778) antibodies were purchased from Santa Cruz Biotechnology (Dallas, Texas, USA). OCT4 (ab19857), NANOG (ab80892) and PFKFB4 (ab137785) antibodies were purchased from Abcam (Cambridge, UK). CD44 (3570), Flag (8146s) and CREB (9197s) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).
3
Immunohistochemical Analysis of Sarcoma Markers
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Commercially available paraffin-embedded tissue array slides containing 79 human sarcomas and 4 human normal tissues (NBP2-30332; Novus Biologicals, and T242, US Biomax, Inc., USA) were purchased. Sections were deparaffinized, then incubated with antibodies recognizing human CD133 (MBS462020; MyBioSource), Nanog (ab80892; Abcam), and p-Akt1/2 (#9271; Cell Signaling) in a solution of PBS with 1% BSA and 0.1% Triton X-100 at 4 °C overnight. Staining was visualized using secondary antibodies tagged with Alexa Fluor 488 (A32766, A10042) and Alexa Fluor 594 (A32744, A32754), from Thermo Fisher, with nuclear counterstaining using DAPI. Images were collected on an inverted confocal microscope (Leica Microsystems) and processed using Imaris 7.6. Five fields were examined for each sample.
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