Digital color fundus photography was performed using the Micron III retinal imaging system (Phoenix Research Laboratories). Prior to fundus imaging, mice were anesthetized with an intraperitoneal injection of 86.9 mg/kg ketamine and 10 mg/kg xylazine and administered 1.0% tropicamide eye drops (Bausch and Lomb) to dilate the pupils.
Tropicamide eye drops
Manufactured by Bausch & Lomb
Sourced in Jersey
Tropicamide eye drops are a topical ophthalmic solution. The active ingredient is tropicamide, which is a mydriatic and cycloplegic agent. Tropicamide eye drops are used to temporarily dilate the pupil and temporarily paralyze the focusing ability of the eye.
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Lab products found in correlation
4 protocols using tropicamide eye drops
1
Retinal Imaging in Anesthetized Mice
2
Subretinal Lentiviral Gene Delivery in Rat Pups
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At the beginning of the 50% O2 cycle on p8, rat pups were given subretinal injections of lentiviral vector stock. General anesthesia was induced by inhalation of isoflurane, eye lids were carefully teased open and pupils were dilated using tropicamide eye drops 1% (Bausch + Lomb, Bridgewater, New Jersey). A 30 G cannula was used to pierce a pilot hole into the sclera near the limbus, a Hamilton syringe with a blunt 33 G cannula was advanced through the vitreous into the subretinal space and 1 µl lentivirus suspension (containing 106 viral particles) in sterile PBS was slowly injected. Animals were randomly assigned to receive bilateral subretinal injections of vehicle (PBS), luciferase shRNA lentivirus (L-lucifshRNA), VEGF164 shRNA lentivirus (L-VEGF164shRNA) or VEGF-A shRNA lentivirus (L-VEGFAshRNA); both eyes received the same treatment. Pups were returned to their dams and replaced into the Oxycycler within 1.5 hours.
3
Fundus Imaging in Anesthetized Mice
4
Intravitreal Injection in Heterozygous rd1 Mice
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Heterozygous L7Cre/+rd1 mice were injected aged 9–10 weeks. Mice were anaesthetised by intraperitoneal injection ketamine (75 mg/kg body weight) and medetomidine (1 mg/kg body weight). Once anaesthetised, mice were positioned on a heat mat to prevent cooling. Pupils were dilated with 1% tropicamide eye drops (Bausch & Lomb) and a 13 mm coverslip was positioned on gel lubricant (Lubrithal) applied to the cornea. Approximately 2.2 μl of virus (1.12 × 1013 genomic counts per ml) was injected into the vitreous of each eye using a Nanofil 10‐μl syringe (World Precision Instruments) using 35‐gauge bevelled needle using a surgical microscope (M620 F20, Leica). All mice received bilateral injections. Anaesthesia was reversed by intraperitoneal injection of atipamezole (3 mg/kg body weight). During recovery, 0.5% bupivacaine hydrochloride and 0.5% chloramphenicol were applied topically to the injected eyes. Mice also received 0.25 ml of warm saline by subcutaneous injection to aid recovery.
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