Wild-type C57BL/6J, SIRT1flox/flox (Stock No. 008041), and Emx1-ires-Cre knockin mice (Stock No. 005628) were purchased from Jackson Laboratory and maintained by breeding colonies. Animals were housed in groups of 3–5 under a 12/12-h light/dark cycle (lights on at 0700 h) with ad libitum access to food and water. Adult male and female mice were used for the experiments. All animal procedures were approved by the Institutional Animal Care and Use Committee of University of Texas Health Science Center at San Antonio and Binzhou Medical University Hospital.
Emx1 ires cre knock in mice
Manufactured by Jackson ImmunoResearch
Emx1-IRES-Cre knock-in mice are a genetically modified mouse line that expresses Cre recombinase under the control of the Emx1 gene promoter. The Emx1 gene is expressed in the dorsal telencephalon, including the cerebral cortex, hippocampus, and some diencephalic structures. The Cre recombinase allows for the conditional, cell-type-specific manipulation of target genes in these brain regions.
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4 protocols using emx1 ires cre knock in mice
1
Genetic Manipulation of SIRT1 in Mice
2
Mouse Line Availability for Study
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No new reagents or materials were generated for this study.
The mouse lines used for this study are available from Jackson Labs (Emx1-IRES-Cre knock-in mice, Jackson Laboratory Stock No: 005628, and NuTRAP mice, Jackson Laboratory Stock No: 029899).
3
Emx1-NuTRAP Mice for Neural Research
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Emx1-IRES-Cre knock-in mice (Jackson Laboratory Stock No: 005628), NuTRAP mice (Jackson Laboratory Stock No: 029899), and C57Bl6/J (Jackson Laboratory Stock No: 000664) wild type mice were obtained from Jackson Laboratories. Female Emx1-IRES-Cre and male NuTRAP mice were crossed to generate the Emx1-NuTRAP mice used for this study. Mice were given free access to food and water, and lights were maintained on a standard 12-h light/dark cycle. On postnatal day (P) 21, mice were weaned and pair-housed in either standard bedding cages or cages equipped with a running wheel. Only male mice were used for the studies performed. Experiments were conducted according to US National Institutes of Health guidelines for animal care and use and were approved by the Institutional Animal Care and Use Committee of the University of California, Irvine.
4
Optogenetic Silencing of Mouse Cortex
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Optogenetic silencing of cortex was performed using Emx1-Halo mice as previously described 27 (link) . Briefly, Emx1-IRES-Cre knock-in mice (Jackson Laboratories, stock #005628) were crossed to Rosalox-stop-lox (RSL)-eNpHR3.0/eYFP mice (Ai39, JAX, stock# 006364), which express halorhodopsin after excision of a stop cassette by Cre recombinase. All mouse lines were maintained on a C57BL/6 background. Optogenetic experiments used mice that were heterozygous for the desired transgene as assessed by in-house genotyping. The locations of S1 and M1 were marked based on stereotaxic coordinates during headplate surgery, and the skull was thinned before recordings. Light was generated by a 593-or 594-nm laser (OEM or Coherent) coupled to a 200-μm diameter, 0.39 NA optic fiber (Thorlabs) via a fiberport, and the diamond-knife cut fiber tip was placed above M1 or S1.
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