Live and fixed cells were imaged under Olympus IX83 inverted wide-field microscope system equipped with an oil ×100/NA 1.40 objective (Plan Apo), an oil ×63/NA 1.40 objective (Plan Apo), and an oil ×40/NA 1.20 objective (fluorite), a motorized stage, a focus drift correction device, a 37 °C enclosed environment chamber, motorized filter cubes, a scientific complementary metal oxide semiconductor camera (Neo; Andor), and a 200-W metal-halide excitation light source (Lumen Pro 200; Prior Scientific). Filters and dichroic mirrors were optimized for GFP/Alexa Fluor 488, mCherry/Alexa Fluor 594, and Alexa Fluor 680. The microscope system was controlled by MetaMorph (Molecular Devices).
Oil 40 na 1.20 objective
Manufactured by Oxford Instruments
The Oil ×40/NA 1.20 objective is a high-performance microscope objective lens designed for use in various laboratory applications. It provides a magnification of 40x and a numerical aperture (NA) of 1.20, which enables the capture of high-resolution images and the observation of fine details. This objective is suitable for use with oil immersion techniques, which can improve optical performance and resolution.
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Lab products found in correlation
2 protocols using oil 40 na 1.20 objective
1
Multimodal Microscopy Imaging Protocol
2
Live-cell Microscopic Imaging Setup
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Live and fixed cells were imaged under Olympus IX83 inverted wide-field microscope system equipped with an oil 100×/NA 1.40 objective (Plan Apo), an oil 63×/NA 1.40 objective (Plan Apo) and an oil 40×/NA 1.20 objective (fluorite), a motorized stage, a focus drift correction device, a 37 °C enclosed environment chamber, motorized filter cubes, a scientific complementary metal oxide semiconductor camera (Neo; Andor) and a 200 W metal-halide excitation light source (Lumen Pro 200; Prior Scientific). Filters and dichroic mirrors were optimized for GFP/Alexa Fluor 488, mCherry/Alexa Fluor 594 and Alexa Fluor 680. The microscope system was controlled by MetaMorph (Molecular Devices).
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