Escherichia coli strain BL21 (DE3) plysS and pET30a vector were obtained from Novagen (La Jolla, CA). All restriction enzymes and ExTaq Polymerase were purchased from TaKaRa Biotechnology (Liaoning China). Water was generated by using a Milli‐Q‐Gradient purification system (Millipore). Medium components tryptone and yeast extract were purchased from Oxoid. Isopropylb‐d‐1‐thiogalactopyranoside (ITPG, >99 %), ampicillin sodium salt (>99 %), kanamycin sulfate (>99 %), and meta‐chloroperbenzoic acid were purchased from Sigma‐Aldrich. Thioanisole was purchased from J&K Scientific and used without further purification. Acetic acid, hexanoic acid, heptanoic acid, octanoic acid, 2‐phenylpropionic acid, n‐butanoicacid, oxone, hydrogen peroxide were purchased form Adamas‐beta. All other chemicals and reagents were purchased from J&K Scientific, China.
Hydrogen peroxide
Manufactured by Adamas
Hydrogen peroxide is a clear, colorless liquid chemical compound with the formula H2O2. It is a common oxidizing agent used in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Ampicillin sodium salt, by Merck Group (1 mentions)
Kanamycin sulfate, by Merck Group (1 mentions)
Ex taq polymerase, by Takara Bio (1 mentions)
Meta chloroperbenzoic acid, by Merck Group (1 mentions)
Milli q gradient purification system, by Merck Group (1 mentions)
Isopropyl b d 1 thiogalactopyranoside, by Merck Group (1 mentions)
Thioanisole, by J&K Scientific (1 mentions)
2 phenylpropionic acid, by Adamas (1 mentions)
Acetic acid, by Adamas (1 mentions)
P benzoquinone, by Maclin Biochemical Technology (1 mentions)
Silicon dioxide, by Maclin Biochemical Technology (1 mentions)
New coccine, by Merck Group (1 mentions)
Methanol, by Sinopharm (1 mentions)
Nitric acid, by Shanghai Chemical Reagent (1 mentions)
2 protocols using hydrogen peroxide
1
Recombinant Protein Expression in E. coli
Check if the same lab product or an alternative is used in the 5 most similar protocols
Escherichia coli strain BL21 (DE3) plysS and pET30a vector were obtained from Novagen (La Jolla, CA). All restriction enzymes and ExTaq Polymerase were purchased from TaKaRa Biotechnology (Liaoning China). Water was generated by using a Milli‐Q‐Gradient purification system (Millipore). Medium components tryptone and yeast extract were purchased from Oxoid. Isopropylb‐d‐1‐thiogalactopyranoside (ITPG, >99 %), ampicillin sodium salt (>99 %), kanamycin sulfate (>99 %), and meta‐chloroperbenzoic acid were purchased from Sigma‐Aldrich. Thioanisole was purchased from J&K Scientific and used without further purification. Acetic acid, hexanoic acid, heptanoic acid, octanoic acid, 2‐phenylpropionic acid, n‐butanoicacid, oxone, hydrogen peroxide were purchased form Adamas‐beta. All other chemicals and reagents were purchased from J&K Scientific, China.
+ Expand
Escherichia coli strain BL21 (DE3) plysS and pET30a vector were obtained from Novagen (La Jolla, CA). All restriction enzymes and ExTaq Polymerase were purchased from TaKaRa Biotechnology (Liaoning China). Water was generated by using a Milli‐Q‐Gradient purification system (Millipore). Medium components tryptone and yeast extract were purchased from Oxoid. Isopropylb‐d‐1‐thiogalactopyranoside (ITPG, >99 %), ampicillin sodium salt (>99 %), kanamycin sulfate (>99 %), and meta‐chloroperbenzoic acid were purchased from Sigma‐Aldrich. Thioanisole was purchased from J&K Scientific and used without further purification. Acetic acid, hexanoic acid, heptanoic acid, octanoic acid, 2‐phenylpropionic acid, n‐butanoicacid, oxone, hydrogen peroxide were purchased form Adamas‐beta. All other chemicals and reagents were purchased from J&K Scientific, China.
2
Synthesis of Functionalized Polymers and Ligands
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+ Expand
tert-Butanol
(≥99%), alcohol (EtOH, 99.7%), manganese(II) nitrate (50 wt
% aqueous solution), and nitric acid (HNO3, 65–68%)
were purchased from the Shanghai Chemical Reagent Company. Tetramethyl
orthosilicate (TMOS, 99.0%), methylene blue trihydrate (MB, 82%),
and hydrogen peroxide (H2O2, 30%) were purchased
from Adamas Reagent. Methanol (CH3OH, 99.5%) was purchased
from Sinopharm Chemical Reagent Co., Ltd. New coccine (75.0%) was
purchased from Sigma-Aldrich. Manganese acetate (Mn(CH3COO)2, 98%), rhodamine 6G (95%), and methyl orange (96%)
were purchased from Aladdin. Orange II sodium salt (>85%), p-benzoquinone (99.0%), and silicon dioxide (99.5%) were
purchased from Macklin. Orange G (96%) was purchased from Meryer.l -Ascorbic acid was purchased from TCI (Shanghai) Development
Co., Ltd. Synthesis of quaternized poly(2-vinylpyridine)-b-poly(ethylene oxide) (positively charged P2MVP128-b-PEO477, Mw/Mn = 1.1, Mw = 34.5k,
level of quaternization is ∼90%) is described in the previous
work.29 (link),30 (link),63 (link) The 1,3,5-tris(2,6-dicarboxypyridin-4-yloxymethyl)benzene
ligands (L3) were synthesized according to the literature.31 (link)
(≥99%), alcohol (EtOH, 99.7%), manganese(II) nitrate (50 wt
% aqueous solution), and nitric acid (HNO3, 65–68%)
were purchased from the Shanghai Chemical Reagent Company. Tetramethyl
orthosilicate (TMOS, 99.0%), methylene blue trihydrate (MB, 82%),
and hydrogen peroxide (H2O2, 30%) were purchased
from Adamas Reagent. Methanol (CH3OH, 99.5%) was purchased
from Sinopharm Chemical Reagent Co., Ltd. New coccine (75.0%) was
purchased from Sigma-Aldrich. Manganese acetate (Mn(CH3COO)2, 98%), rhodamine 6G (95%), and methyl orange (96%)
were purchased from Aladdin. Orange II sodium salt (>85%), p-benzoquinone (99.0%), and silicon dioxide (99.5%) were
purchased from Macklin. Orange G (96%) was purchased from Meryer.
Co., Ltd. Synthesis of quaternized poly(2-vinylpyridine)-b-poly(ethylene oxide) (positively charged P2MVP128-b-PEO477, Mw/Mn = 1.1, Mw = 34.5k,
level of quaternization is ∼90%) is described in the previous
work.29 (link),30 (link),63 (link) The 1,3,5-tris(2,6-dicarboxypyridin-4-yloxymethyl)benzene
ligands (L3) were synthesized according to the literature.31 (link)
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