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Anti sca 1 d7

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Anti-Sca-1(D7) is a laboratory reagent used for research purposes. It is an antibody that binds to the Sca-1 (Stem Cell Antigen-1) protein, which is expressed on the surface of certain types of stem and progenitor cells. The core function of Anti-Sca-1(D7) is to enable the identification, isolation, and study of Sca-1-positive cell populations in various research applications.

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Lab products found in correlation

Moflo astrios cell sorter, by Beckman Coulter (1 mentions) Streptavidin bv421, by BioLegend (1 mentions) Streptavidin apc efluor 780, by Thermo Fisher Scientific (1 mentions) Facsaria flow cytometer, by BD (1 mentions) Ghost dye red 780, by Cytek Biosciences (1 mentions) Goat anti lepr biotin af497, by R&D Systems (1 mentions) Facsdiva 8, by BD (1 mentions) Prolong gold, by Thermo Fisher Scientific (1 mentions) Anti ki67 sola15, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde solution, by Electron Microscopy Sciences (1 mentions) Hc pl apo 40x 1.3 oil, by Leica (1 mentions) Anti cd8α clone 53 6, by Thermo Fisher Scientific (1 mentions) Tcs sp8 confocal microscope, by Leica (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)

Close spelling variants of this product

PE-Cy7 anti-mouse Ly6A/E (Sca-1) (clone D7) Anti-Sca-1-APC (clone D7 at PE)-Cy5 anti-mouse Ly-6A/E (Sca-1) (clone D7; PE-Cy7-anti-Sca-1 (D7, Sca-1 (D7) Anti-Sca-1 Sca-1

4 protocols using anti sca 1 d7

1

Isolation and Characterization of Adipose SVF

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The stromal vascular fraction was separated from adipocytes by centrifugation after digestion. Excised white adipose tissue was digested in RPMI with 0.5% BSA and 1 mg ml−1 type II collagenase for 30 min at 37 °C with gentle agitation. Then the cell suspension was filtered through a 100 μm filter and centrifuged at 700 g for 5 min to separate floating adipocytes from SVC pellet, and RPMI with 0.5% BSA was used to wash cells. The following antibodies were used for staining: anti-CD45(30-Fll), anti-CD31(390), anti-CDllc(N418) and anti-Sca-1(D7), all from eBioscience. Stained SVC were sorted by flow cytometry on a MoFlo Astrios cell sorter (Beckman Coulter) with the help of the University of Michigan's Flow Cytometry Core.
Reilly S.M., Ahmadian M., Zamarron B.F., Chang L., Uhm M., Poirier B., Peng X., Krause D.M., Korytnaya E., Neidert A., Liddle C., Yu R.T., Lumeng C.N., Oral E.A., Downes M., Evans R.M, & Saltiel A.R. (2015). A subcutaneous adipose tissue–liver signalling axis controls hepatic gluconeogenesis. Nature Communications, 6, 6047.
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2

Stromal Vascular Fraction Isolation

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The stromal vascular fraction was separated from adipocytes by centrifugation after digestion. Excised white adipose tissue was digested in RPMI with 0.5% BSA and 1 mg ml−1 type II collagenase for 30 min at 37 °C with gentle agitation. Then the cell suspension was filtered through a 100 μm filter and centrifuged at 700 g for 5 min to separate floating adipocytes from SVC pellet, and RPMI with 0.5% BSA was used to wash cells. The following antibodies were used for staining: anti-CD45(30-F11), anti-CD31(390), anti-CD11c(N418) and anti-Sca-1(D7), all from eBioscience. Stained SVC were sorted by flow cytometry on a MoFlo Astrios cell sorter (Beckman Coulter) with the help of the University of Michigan’s Flow Cytometry Core.
Reilly S.M., Ahmadian M., Zamarron B.F., Chang L., Uhm M., Poirier B., Peng X., Krause D.M., Korytnaya E., Neidert A., Liddle C., Yu R.T., Lumeng C.N., Oral E.A., Downes M., Evans R.M, & Saltiel A.R. (2015). A subcutaneous adipose tissue–liver signalling axis controls hepatic gluconeogenesis. Nature Communications, 6, 6047.
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3

Flow Cytometry Staining of Biomarkers

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Samples were stained with combinations of the following antibodies for flow cytometry: Goat-anti-LepR-biotin (AF497, R&D Systems), anti-CD45 (30F-11, Tonbo Biosciences), anti-CD144 (clone BV13, eBiosciences), anti-TER119 (Tonbo Biosciences), anti-Sca1(D7, eBiosciences), anti-CD51-biotin (RMV-7, BioLegend), Streptavidin BV421 (BioLegend), and/or Streptavidin APCeFluor 780 (eBiosciences). All staining was performed for 1.5 h on ice. Dead cells were identified and gated out of sorts by including 4 0 ,6-diamidino-2-phenylindole (DAPI) (1 mg/mL) in the buffer in which cells were resuspended for flow cytometry or by staining cells with Ghost Dye Red 780 (1:100, Tonbo Biosciences) before resuspending cells for flow cytometric analysis. Samples were analyzed or sorted using FACSAria flow cytometers and FACSDiva 8.0 (BD) or FlowJo v10.6.1 (Tree Star) software.
Jeffery E.C., Mann T.L.A., Pool J.A., Zhao Z, & Morrison S.J. (2022). Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair. Cell stem cell, 29(11).
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4

Immunofluorescence Imaging of Mouse Ear Pinnae

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Ear pinnae were split with forceps, fixed in 1% paraformaldehyde solution (Electron Microscopy Sciences) overnight at 4°C and blocked in 1% BSA, 0.25% Triton X blocking buffer for 2 hours at room temperature. Tissues were first stained with anti-CD8α (clone 53-6.7, eBioscience), anti-Sca-1 (D7, eBioscience) and/or rabbit anti-CD31 (390, eBioscience), and anti-Ki67 (solA15, eBioscience) antibodies overnight at 4°C, washed three time with PBS and then stained with 4′, 6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich) for 5 min at room temperature and before being mounted with ProLong Gold (Molecular Probes) antifade reagent. Ear pinnae images were captured on a Leica TCS SP8 confocal microscope with a 40X oil objective (HC PL APO 40X/1.3 oil). Images were analyzed using Imaris Bitplane software.
Linehan J.L., Harrison O.J., Han S.J., Byrd A.L., Vujkovic-Cvijin I., Villarino A.V., Sen S.K., Shaik J., Smelkinson M., Tamoutounour S., Collins N., Bouladoux N., Dzutsev A., Rosshart S.P., Arbuckle J.H., Wang C.R., Kristie T.M., Rehermann B., Trinchieri G., Brenchley J.M., O’Shea J.J, & Belkaid Y. (2018). Non-classical immunity controls microbiota impact on skin immunity and tissue repair. Cell, 172(4), 784-796.e18.
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