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Alexa 594

Manufactured by IDEX Corporation
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Alexa 594 is a fluorescent dye commonly used in flow cytometry, immunocytochemistry, and other fluorescence-based applications. It has an excitation maximum at 594 nm and an emission maximum at 624 nm, making it compatible with common laser and filter sets. The dye is known for its brightness and photostability, allowing for sensitive and reliable detection of target molecules or cells.

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Lab products found in correlation

Filter cubes, by IDEX Corporation (2 mentions) Spectra x light engine, by Lumencor (2 mentions) Ti e epifluorescence microscope, by Lumencor (2 mentions) Zyla 5.5 scmos camera, by Oxford Instruments (2 mentions) Alexa 488, by IDEX Corporation (2 mentions) Gfp 1828a nte zero, by IDEX Corporation (2 mentions) Quasar 570, by IDEX Corporation (2 mentions)

2 protocols using alexa 594

1

Multi-Omics Imaging of Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Both cultured cells as well as LabelX treated and expanded cultured cells were imaged on a Nikon Ti-E epifluorescence microscope with a SPECTRA X light engine (Lumencor), and a 5.5 Zyla sCMOS camera (Andor), controlled by NIS-Elements AR software. For Fig. 1c, 1d, and Supplementary Fig 3–5 a 40× 1.15 NA water immersion objective was used. For all other experiments with cultured cells, a 60× 1.4 NA oil immersion objective was used.
For imaging smFISH probes labeled with fluorophores, the following filter cubes (Semrock, Rochester, NY) were used: Alexa 488, GFP-1828A-NTE-ZERO; Quasar 570, LF561-B-000; Alexa 594, FITC/TXRED-2X-B-NTE; Atto 647N, Cy5-4040C-000.
Chen F., Wassie A.T., Cote A.J., Sinha A., Alon S., Asano S., Daugharthy E.R., Chang J.B., Marblestone A., Church G.M., Raj A, & Boyden E.S. (2016). Nanoscale Imaging of RNA with Expansion Microscopy. Nature methods, 13(8), 679-684.
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2

Multi-Omics Imaging of Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Both cultured cells as well as LabelX treated and expanded cultured cells were imaged on a Nikon Ti-E epifluorescence microscope with a SPECTRA X light engine (Lumencor), and a 5.5 Zyla sCMOS camera (Andor), controlled by NIS-Elements AR software. For Fig. 1c, 1d, and Supplementary Fig 3–5 a 40× 1.15 NA water immersion objective was used. For all other experiments with cultured cells, a 60× 1.4 NA oil immersion objective was used.
For imaging smFISH probes labeled with fluorophores, the following filter cubes (Semrock, Rochester, NY) were used: Alexa 488, GFP-1828A-NTE-ZERO; Quasar 570, LF561-B-000; Alexa 594, FITC/TXRED-2X-B-NTE; Atto 647N, Cy5-4040C-000.
Chen F., Wassie A.T., Cote A.J., Sinha A., Alon S., Asano S., Daugharthy E.R., Chang J.B., Marblestone A., Church G.M., Raj A, & Boyden E.S. (2016). Nanoscale Imaging of RNA with Expansion Microscopy. Nature methods, 13(8), 679-684.
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