Phospho specific anti eif2α

Phospho-specific anti-eIF2α, anti-eIF2α, anti-eIF4GI, anti-4EBP1 were obtained from Cell Signaling Technology (Beverly, MA). Anti-DDX3 and anti-tubulin antibodies were purchased from Abcam, and anti-ATF4 antibody from Santa Cruz Biotech. Anti-eIF4E was obtained from BD Bioscience. Anti-HuR and anti-FMRP antibodies were previously described^{15 (link),53 (link)}.

Phospho-specific anti-eIF2α and the pan anti-eIF2 were purchased from Cell Signaling Technology (Beverly, MA). Anti-FMRP, anti-FXR1, anti-eIF4E, anti-eIF4GI and anti-G3BP1 antibodies have been previously described [3 (link)]. Anti-RCK, anti-ATF4, and anti-HRI antibodies were purchased from Santa Cruz Biotech. Anti-PERK and anti-tubulin antibodies were obtained from Abcam. Anti-puromycin antibodies were obtained from EMD Millipore.

Anti-FMRP, anti-FXR1 and anti-G3BP antibodies have been previously described [22 (link)]. Anti-eIF4GI, phospho-specific anti-eIF2α, the pan anti-eIF2α, anti-4EBP1 and anti-phospho-4EBP1 were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-DDX3, -PERK, -GCN2 and -α-tubulin antibodies were purchased from Abcam. Anti-m [6 (link)] A antibody was obtained from Synaptic Systems (Germany). Anti-HRI antibodies were purchased from Santa Cruz Biotech (Santa Cruz, CA, USA). Anti-puromycin antibody was obtained from EMD Millipore (Merck, Germany). Anti-Vinculin was provided by Dr. M-E Huot (Université Laval). Anti-Dcp1a and anti-LSM14A have been described previously [23 (link),24 (link)].