Kolliphor hs 15 hs 15

Primary antibodies used in this study were detailed in Table S1. Cell lysis buffer (Cat. 9803) were from Cell Signaling Technology. Recombinant human or mouse IL6 (Cat. 216‐16; 200‐06), TNFα (Cat. 315‐01A; 300‐01A) and IFNβ (Cat. 300‐02BC) were obtained from PeproTech, whereas protease inhibitor (Cat. 11836145001) and phosphorylation inhibitor cocktail (Cat. 4906837001) tablets were from Roche Diagnostics. Horseradish peroxidase‐conjugated secondary antibodies (Cat. abs20001; abs20002) were from Absin. Kolliphor® HS 15 (HS‐15) (Cat. 42966), and LPS (Cat. 916374) were bought from Sigma. Mouse granulocyte colony‐stimulating factor (G‐CSF) ELISA Kit (Cat. ab197743) was bought from Abcam. The chemical libraries were purchased from TargetMol or The National Center for Drug Screening (China). The detailed compound information was listed in Table S2. The scaffold compound L971‐0101 (L971) was acquired from TargetMol.

Primary antibodies used in the study were detailed in Table S1. Cell lysis buffer (Cat. 9803) was purchased from Cell Signaling Technology. Protease Inhibitor (Cat. 11836145001), Phosphorylation Inhibitor Cocktail Reagent (Cat. 4906837001) and SYBR Green PCR Master Mix (2×) (Cat. 4913914001) were from Roche Diagnostics. Horseradish peroxidase-conjugated secondary antibodies (Cat. abs20001; abs20002) were obtained from Absin. Kolliphor^® HS 15 (HS-15) (Cat. 42966) and lipopolysaccharide/LPS (Cat. 916374) were from Sigma. Recombinant mouse IL-6 (Cat. 200-06), human and mouse TNF-α (Cat. 315-01A; 300-01A), mouse IFN-γ (Cat. 315-05) and IFN-β (Cat. 300-02BC) were obtained from PeproTech. Mouse granulocyte colony-stimulating factor (G-CSF) ELISA Kit (Cat. ab197743) was bought from Abcam. Mouse IL-6 ELISA Kit (Cat. abs520004) and Mouse IL-1β ELISA Kit (Cat. abs520001) were purchased from Absin. Urea Assay Kit (Cat. C013-2-1), Alanine aminotransferase (ALT) Assay Kit (Cat. C009-2-1), and Aspartate aminotransferase (AST) Assay Kit (Cat. C010-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute. The detailed compound library information was as in our previously published literature (14 (link)). The bioactive compound C498-0670 (C498) was acquired from TargetMol.