E600003

Normal cervical cell line HcerEpic and human cervical cancer cell lines, including CaSki, C‐33A HeLa, HeLa‐229, ME‐180, MS751 and SiHa, were obtained from National Collection of Authenticated Cell Cultures of China. All cell lines were maintained in DMEM (E600003; Sangon Biotech) containing 10% foetal bovine serum (E510008; Sangon Biotech) in a 5% CO₂ atmosphere at 37°C.

The murine breast cancer 4T1 (Cell Bank of the Chinese Academy of Sciences, China) was cultured in the complete RPMI‐1640 medium (Sangon Biotech; E600028) including 10% fetal bovine serum (FBS; Excell Bio, Shanghai; FSP500) and 1% penicillin–streptomycin 100× (PS; Macgene, Beijing, CC004). The murine melanoma B16F10 (Cell Bank of the Chinese Academy of Sciences, China) was cultured in the complete DMEM medium (high glucose; Sangon Biotech; E600003) including 10% FBS and 1% penicillin–streptomycin 100×. The murine kidney cancer Renca was purchased from Procell (Wuhan; CL‐0568) and incubated with complete RPMI‐1640 (Procell, Wuhan; PM150110) with 10%FBS, 1% penicillin–streptomycin 100x, 1% MEM Nonessential Amino Acids Solution 100× (NEAA; Macgene; CC25025), 1 mM Sodium Pyruvate (Macgene; CC007), and 2 mM L‐glutamine (Macgene; CC009). The human normal endothelial cell line PUMC‐HUVEC‐T1 was cultured in the DMEM medium (high glucose) and added 1%NEAA and insulin (Macgene).
All the cells were incubated in their special medium and maintained in a carbon dioxide cell culture box (Thermo Fisher‐Forma 371) with 5% CO₂ at 37°C.