Smarter race 3 kit

Manufactured by Takara Bio

Sourced in China

The SMARTer RACE 3' kit is a laboratory product developed by Takara Bio. It is designed to facilitate rapid amplification of cDNA 3' ends using a simple and efficient protocol.

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Lab products found in correlation

Nucelospin gel and pcr clean up kit, by Takara Bio (1 mentions)

Spelling variants of this product

SMARTer RACE kit (59 citations) RACE kit (24 citations) 3′RACE kit (5 citations)

2 protocols using smarter race 3 kit

Full-length PLAG1 3'-UTR Amplification

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A SMARTer RACE 3′ kit (Takara, Nanjing, China) was used to amplify the end sequence of PLAG1 3′-UTR. The 3′ RACE method combined with nested polymerase chain reaction (PCR) was used to obtain the full-length PLAG1 3′-UTR. The 3′ RACE protocol was as follows: the first round of the nested PCR protocol was at 95 °C for 3 min, followed by 30 cycles at 95 °C for 15 s, 68 °C for 15 s, and 72 °C for 3 min, and a final round at 72 °C for 5 min; the second round of the nested PCR protocol was at 95 °C for 3 min, followed by 30 cycles at 95 °C for 15 s, 56 °C for 15 s, and 72 °C for 3 min, and a final round at 72 °C for 5 min. Amplification was visualized using 1.5% agarose gel electrophoresis and ligated into pMD19-T vector and transformation, and then the bacterial liquid was sequenced (Tsingke, Beijing, China).

Wang Y., Li Y.X., Zhang J., Qian Y., Meng C.H., Zhong J.F, & Cao S.X. (2023). PLAG1 g.8795C>T Mutation Regulates Early Body Weight in Hu Sheep by Weakening miR-139 Binding. Genes, 14(2), 467.

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3'-RACE for TTLL12 cDNA Amplification

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As displayed in Fig. 1, the full length of TTLL12 cDNA was amplified by 3′-RACE (SMARTer RACE 3′ kit; Takara, Dalian, China). First-strand cDNA synthesis from total RNA of H1299 was performed using a traditional reverse transcription procedure, but with a special oligo(dT) primer: [3′-RACE CDS primer, 5′-AAGCAGTGGTATCAACGCAGAGTAC (T) 30VN-3′; N=A, C, G or T; V=A, G, or C]. The first-strand cDNA synthesis reaction products were diluted with 10 µl Tricine-EDTA Buffer (Takara). The diluted first-strand cDNA was used as template and second-strand synthesis was amplified with a 3′ gene-specific primer (3′ GSP, 5′-GATTACGCCAAGCTTAGAGCACACAGACGGCGCGGGTG-3′) and universal primer mix A (UPM; long, 5′-CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT-3′ and short, 5′-CTAATACGACTCACTATAGGGC-3′). The 3′-RACE DNA samples were electrophoresed on an agarose gel. The position of the desired fragment was located under UV light and the products were extracted with the NuceloSpin Gel and PCR Clean-Up kit (Takara).

Wen R., Xiao Y., Zhang Y., Yang M., Lin Y, & Tang J. (2016). Identification of a novel transcript isoform of the TTLL12 gene in human cancers. Oncology Reports, 36(6), 3172-3180.

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