Bcl 2 antibody n 19

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Bcl-2 antibody (N-19) is a primary antibody used in research applications. It is specific for the N-terminus of the Bcl-2 protein, which is a key regulator of apoptosis (programmed cell death). The antibody can be used to detect and study the expression of Bcl-2 in various cell and tissue samples.

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Lab products found in correlation

Dynabead, by Thermo Fisher Scientific (1 mentions) Anti β actin antibody c4, by Santa Cruz Biotechnology (1 mentions)

2 protocols using bcl 2 antibody n 19

Immunoprecipitation of Beclin-1 with Bcl-2 and Bcl-xL

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THP-1 macrophages were lysed with a nonionic detergent (Nonidet P-40) to preserve protein-protein binding (36 (link)) and incubated with either agarose-conjugated Bcl-2 antibody (N-19, Santa Cruz Biotechnology), rabbit polyclonal anti-Bcl-xL antibody plus Dynabeads (Life Technologies), or nonimmune rabbit IgG. Immunoprecipitates were immunoblotted using rabbit anti-Beclin 1 antibody conjugated with HRP (Novus Biologicals), and chemiluminescence was assessed as indicated above. Beclin-1 was not detectable in samples immunoprecipitated with rabbit IgG (results not shown).

Casalino-Matsuda S.M., Nair A., Beitel G.J., Gates K.L, & Sporn P.H. (2015). Hypercapnia Inhibits Autophagy and Bacterial Killing in Human Macrophages by Increasing Expression of Bcl-2 and Bcl-xL. Journal of immunology (Baltimore, Md. : 1950), 194(11), 5388-5396.

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Immunoblotting Analysis of Apoptosis Markers

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Anti-β-Actin Antibody (C4) (Santa Cruz Biotechnology, USA), Anti-Bax Antibody (B-9) (Santa Cruz Biotechnology, USA), Bcl-2 Antibody (N-19) (Santa Cruz Biotechnology, USA) were used as primary antibodies for immunoblotting and western blot was performed as previously described 32 (link).

Safari F., Rayat Azad N., Alizadeh Ezdiny A., Pakizehkar S., Khazaei Koohpar Z, & Ranji N. (2022). Antitumor Activities of Green Tea by Up-regulation of miR-181a Expression in LNCaP Cells Using 3D Cell Culture Model. Avicenna Journal of Medical Biotechnology, 14(1), 89-94.

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