Approximately 100 μl of blood was collected in 200 μl of ACD buffer (Becton Dickinson) and processed to remove all red blood cells prior to lymphocyte staining. Cells were treated with TruStain FcX affinity purified anti-mouse CD16/32 Fc block (BioLegend) and stained with DYKDDDDK (FLAG) Tag specific FITC-anti-Flag M2 mAb (Sigma Cat. #F4049) and PE-anti-mouse CD3e mAb (eBioscience Cat. #eBio500A2). Cell samples were analyzed on a BD FACSCaliber cytometer (BD Biosciences).
Tag specific fitc anti flag m2 mab
Manufactured by Merck Group
The FITC-anti-Flag M2 mAb is a fluorescein isothiocyanate (FITC) conjugated monoclonal antibody that specifically binds to the FLAG epitope tag. It is a useful tool for the detection and purification of FLAG-tagged proteins in various applications, such as immunoprecipitation, Western blotting, and flow cytometry.
Automatically generated - may contain errors
2 protocols using tag specific fitc anti flag m2 mab
1
Identification of Activated T Cells via Flow Cytometry
2
Identification of Activated T Cells via Flow Cytometry
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