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Rat monoclonal anti c3 clone 11h9

Manufactured by Abcam
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Rat monoclonal anti C3 (clone 11H9) is a laboratory research antibody that binds to the C3 complement protein. This antibody is for research use only and its core function is to detect the presence and levels of C3 in biological samples.

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Lab products found in correlation

Psd 95, by Thermo Fisher Scientific (2 mentions) Sp clone svp38, by Merck Group (2 mentions) Vglut1, by Merck Group (1 mentions)

Spelling variants of this product

Anti-C3 (7 citations) Rat anti-C3 (4 citations)

2 protocols using rat monoclonal anti c3 clone 11h9

1

Detailed Immunostaining Protocol for Synaptic Markers

Cited 1 time
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The following primary antibodies were used for this study: mouse monoclonal anti‐synaptophysin (SP) (clone SVP38, 1.4 μg/ml, Sigma), guinea pig polyclonal anti VGlut1 (1:1000, Millipore), rabbit polyclonal anti postsynaptic density 95 (PSD95, 1 μg/ml, Invitrogen), rat monoclonal anti CD68 (clone FA‐11, 1.4 μg/ml, Bio‐Rad), rabbit polyclonal anti Iba‐1 (1:1000, Wako), rat monoclonal anti C3 (clone 11H9, 0.5ug/ml, Abcam), and rabbit monoclonal anti C1q (clone 27.1, hybridoma supernatant previously described
40 (link),
41 (link)). The specificity of the anti‐mouse C1q antibody was demonstrated by the lack of C1q staining detected in the hippocampal CA1‐SR of an Arctic C1q conditional KO (ArcC1qaFL/FL:Cx3cr1ERT2) compared with the Arc C1qaFL/FL at super‐resolution level (Supp Figure S2) as characterized previously.
40 (link)
Gomez‐Arboledas A., Fonseca M.I., Kramar E., Chu S., Schartz N.D., Selvan P., Wood M.A, & Tenner A.J. (2024). C5aR1 signaling promotes region‐ and age‐dependent synaptic pruning in models of Alzheimer's disease. Alzheimer's & Dementia, 20(3), 2173-2190.
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2

Antibody Profiling for Synaptic and Immune Markers

Cited 1 time
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The following primary antibodies were used for this study: mouse monoclonal anti synaptophysin (SP) (clone SVP38, 1.4 ug/ml, Sigma), guinea pig polyclonal anti VGlut11 (1:1000, Millipore), rabbit polyclonal anti postsynaptic density 95 (PSD95, 1 ug/ml, Invitrogen), rat monoclonal anti CD68 (clone FA-11, 1.4 ug/ml, Bio-Rad), rabbit polyclonal anti Iba-1 (1:1000, Wako), rat monoclonal anti C3 (clone 11H9, 0.5ug/ml, Abcam) and rabbit monoclonal anti C1q (clone 27.1, hybridoma supernatant previously described (41 (link), 42 (link))). The specificity of the anti-mouse C1q antibody was demonstrated by the lack of C1q staining detected in the hippocampal CA1-SR of an Arctic C1q conditional KO (ArcC1qaFL/FL :Cx3cr1ERT2) compared with the Arc C1qaFL/FL at super-resolution level (Supp Figure 2) and characterized previously (41 (link)).
Gomez-Arboledas A., Fonseca M.I., Kramar E., Chu S.H., Schartz N., Selvan P., Wood M.A, & Tenner A.J. (2023). C5aR1 signaling promotes region and age dependent synaptic pruning in models of Alzheimer’s Disease. bioRxiv.
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