The largest database of trusted experimental protocols

Phosphate buffered saline (pbs)

Manufactured by EUROIMMUN
Sourced in Germany

PBS (Phosphate-Buffered Saline) is a commonly used buffer solution in laboratory settings. Its core function is to maintain a stable pH and osmotic environment for various biological applications, such as cell culture, immunoassays, and biochemical reactions. PBS helps to preserve the structure and function of biomolecules and cells during processing and analysis.

Automatically generated - may contain errors

2 protocols using phosphate buffered saline (pbs)

1

Immunophenotyping of NK and CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
To identify NK and CD8+ T cells subsets, Blood samples (about 10 ml) were
collected in EDTA-containing tubes. The peripheral blood mononuclear cells (PBMCs) were
first isolated using Ficoll-Hypaque (Innotrain, Germany) density gradient centrifugation
(1000×g at room temperature) and then washed two times with phosphate-buffered saline
(PBS, Euroimmun, Germany). Centrifugation of samples was at 300 ×g and 4°C. Subsequently,
isolated PBMCs (106 cells/100 µl) were stained with anti-CD8-APC, CD45RO-PE,
CD27-Percp-eFlour780, CD28-Pe-Cy7, CD57-FITC, and CD56-PerCp-eFlour710 monoclonal
antibodies (mAbs) (all from eBioscience, USA) and incubated at 4°C for 30 minutes. The
samples were fixed with formaldehyde and analyzed within 24 hours by flow cytometer (BD
FACSAria, USA). At least 50,000 events were counted for each sample.
+ Open protocol
+ Expand
2

Spleen Cell Isolation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were killed by anesthesia using ketamine and
xylazine combination. It is 100 mg/kg body weight
for Ketamine and 10 mg/kg body weight for Xylazine
injected i.p. Single-cells were isolated from the spleens
and the erythrocytes were lysed using an isotonic solution
of ammonium chloride (pH=7.2). After washing with
phosphate-buffered saline (PBS, Euroimmun, Germany),
the cell suspensions were used for both phenotypic
analysis and cytokine evaluation.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!